Organic Hyperbolic Material Assisted Illumination Nanoscopy

Autor:	Junxiang Zhao, Steven Edward Bopp, Zhaowei Liu, Sui Yang, Zhaoyu Nie, Jeongho Ha, Chengyu Song, Yeon Ui Lee, Shilong Li, Gde Bimananda Mahardika Wisna, Xiang Zhang, Clara Posner, Jin Zhang
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	Fluorophore Materials science General Chemical Engineering Science General Physics and Astronomy Medicine (miscellaneous) structured illumination microscopy Biochemistry Genetics and Molecular Biology (miscellaneous) organic hyperbolic materials poly(3-hexylthiophenes) Speckle pattern chemistry.chemical_compound poly(3‐hexylthiophenes) super-resolution microscopy Microscopy Nanotechnology General Materials Science Ohm bioimaging Research Articles Lighting business.industry Super-resolution microscopy Bandwidth (signal processing) Resolution (electron density) General Engineering Equipment Design super‐resolution microscopy chemistry Microscopy Fluorescence Optoelectronics Spatial frequency business Research Article
Zdroj:	Advanced Science Advanced Science, Vol 8, Iss 22, Pp n/a-n/a (2021) Advanced science (Weinheim, Baden-Wurttemberg, Germany), vol 8, iss 22
ISSN:	2198-3844
Popis:	Resolution capability of the linear structured illumination microscopy (SIM) plays a key role in its applications in physics, medicine, biology, and life science. Many advanced methodologies have been developed to extend the resolution of structured illumination by using subdiffraction‐limited optical excitation patterns. However, obtaining SIM images with a resolution beyond 40 nm at visible frequency remains as an insurmountable obstacle due to the intrinsic limitation of spatial frequency bandwidth of the involved materials and the complexity of the illumination system. Here, a low‐loss natural organic hyperbolic material (OHM) that can support record high spatial‐frequency modes beyond 50k 0, i.e., effective refractive index larger than 50, at visible frequencies is reported. OHM‐based speckle structured illumination microscopy demonstrates imaging resolution at 30 nm scales with enhanced fluorophore photostability, biocompatibility, easy to use and low cost. This study will open up a new route in super‐resolution microscopy by utilizing OHM films for various applications including bioimaging and sensing. The developed organic hyperbolic material offers an alternative path to controlling light at the nanoscale, leading to a significant resolution improvement in biological cell imaging. Organic hyperbolic material assisted illumination nanoscopy shows that precision of lateral resolution reaches down to 30 nm, which is more than 17 times spatial resolution improvement beyond the diffraction limit.
Databáze:	OpenAIRE
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