Automated image analysis of a glomerular injury marker desmin in spontaneously diabetic Torii rats treated with losartan
| Autor: | Kinya Okada, Hiroyuki Utsumi, Yoshihiro Hirohashi, Mizue Kawai, Keisuke Fujitaka, Tsuyoshi Kato, Raissa Relator, Atsushi Fukunari, Tetsuhiro Kakimoto, Taku Iguchi, Masashi Nishio |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Blood Glucose
Male medicine.medical_specialty Angiotensin receptor Endocrinology Diabetes and Metabolism Kidney Glomerulus Type 2 diabetes urologic and male genital diseases Losartan Rats Mutant Strains Desmin Nephropathy Podocyte Rats Sprague-Dawley Diabetic nephropathy Endocrinology Internal medicine Diabetes mellitus Image Processing Computer-Assisted medicine Animals Diabetic Nephropathies Podocytes business.industry Body Weight medicine.disease Rats Disease Models Animal medicine.anatomical_structure Diabetes Mellitus Type 2 business Angiotensin II Type 1 Receptor Blockers Biomarkers medicine.drug |
| Zdroj: | Journal of Endocrinology. 222:43-51 |
| ISSN: | 1479-6805 0022-0795 |
| Popis: | Diabetic nephropathy is a major complication in diabetes and a leading cause of end-stage renal failure. Glomerular podocytes are functionally and structurally injured early in diabetic nephropathy. A non-obese type 2 diabetes model, the spontaneously diabetic Torii (SDT) rat, is of increasing preclinical interest because of its pathophysiological similarities to human type 2 diabetic complications including diabetic nephropathy. However, podocyte injury in SDT rat glomeruli and the effect of angiotensin II receptor blocker treatment in the early stage have not been reported in detail. Therefore, we have evaluated early stages of glomerular podocyte damage and the beneficial effect of early treatment with losartan in SDT rats using desmin as a sensitive podocyte injury marker. Moreover, we have developed an automated, computational glomerulus recognition method and illustrated its specific application for quantitatively studying glomerular desmin immunoreactivity. This state-of-the-art method enabled automatic recognition and quantification of glomerular desmin-positive areas, eliminating the need to laboriously trace glomerulus borders by hand. The image analysis method not only enabled assessment of a large number of glomeruli, but also clearly demonstrated that glomerular injury was more severe in the juxtamedullary region than in the superficial cortex region. This applied not only in SDT rat diabetic nephropathy but also in puromycin aminonucleoside-induced nephropathy, which was also studied. The proposed glomerulus image analysis method combined with desmin immunohistochemistry should facilitate evaluations in preclinical drug efficacy studies as well as elucidation of the pathophysiology of diabetic nephropathy. |
| Databáze: | OpenAIRE |
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