Sequence-Dependent Stability of Intramolecular DNA Triple Helices
| Autor: | Klaus Weisz, Dietmar Leitner |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Magnetic Resonance Spectroscopy
Pyrimidine Ultraviolet Rays Temperature Protonation DNA General Medicine Hydrogen-Ion Concentration Cytosine chemistry.chemical_compound Crystallography Models Chemical chemistry Structural Biology Duplex (building) Intramolecular force Nucleic Acid Conformation Thermal stability Molecular Biology Triple helix |
| Zdroj: | Journal of Biomolecular Structure and Dynamics. 17:993-1000 |
| ISSN: | 1538-0254 0739-1102 |
| DOI: | 10.1080/07391102.2000.10506587 |
| Popis: | A set of 21 oligodeoxynucleotides were designed to fold into intramolecular triple helices of the pyrimidine motif under appropriate conditions. UV melting experiments on the triplexes which only differ in the number and distribution of third strand cytosines reveal the influence of sequence and pH on triplex stability and can be summarized as follows: (1) increasing the cytosine content in the third strand results in a higher thermal stability of the triplex at acidic pH but lowers the triplex to duplex melting temperature at neutral pH; (2) cytosines at terminal positions destabilize the triple helical structure as compared to non-terminal positions; (3) contiguous cytosines lead to a pH dependent destabilization of the triplex, the destabilizing effect being more pronounced at higher pH. Analysis of these effects in terms of the various interactions within a triple helical complex indicate that the sequence-dependent stabilities are largely determined by the extent of protonation for individual third strand cytosines. |
| Databáze: | OpenAIRE |
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