Differential Diagnosis of Pulmonary Carcinoma Following Head and Neck Cancer by Genetic Analysis
| Autor: | N. van Zandwijk, M.L.F. van Velthuysen, T. Hooft van Huysduynen, R.H. Brakenhoff, Petra M. Nederlof, M.W.M. van den Brekel, H. van Tinteren, A.J.M. Balm, T.W. Geurts, F. Broekman |
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| Přispěvatelé: | Ear, Nose and Throat, Otolaryngology / Head & Neck Surgery, CCA - Oncogenesis |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Cancer Research Pathology medicine.medical_specialty Lung Neoplasms endocrine system diseases Concordance DNA Mutational Analysis Loss of Heterozygosity Diagnosis Differential Loss of heterozygosity medicine Carcinoma Humans Neoplasm Metastasis neoplasms Aged Aged 80 and over business.industry Head and neck cancer Cancer Neoplasms Second Primary Gold standard (test) Middle Aged Genes p53 medicine.disease Oncology Head and Neck Neoplasms Carcinoma Squamous Cell Mutation testing Female Differential diagnosis business Algorithms |
| Zdroj: | Clinical cancer research, 15(3), 980-985. American Association for Cancer Research Inc. Geurts, T, van Velthuysen, M L F, Broekman, F, van Huysduynen, T H, van den Brekel, M W M, van Zandwijk, N, van Tinteren, H, Nederlof, PM, Balm, A J & Brakenhoff, R H 2009, ' Differential Diagnosis of Pulmonary Carcinoma Following Head and Neck Cancer by Genetic Analysis ', Clinical Cancer Research, vol. 15, no. 3, pp. 980-985 . https://doi.org/10.1158/1078-0432.CCR-08-1968 Clinical Cancer Research, 15(3), 980-985. American Association for Cancer Research Inc. |
| ISSN: | 1078-0432 |
| DOI: | 10.1158/1078-0432.CCR-08-1968 |
| Popis: | Purpose: Patients with head and neck cancer often develop a lung tumor that can be diagnosed as distant metastasis (DM) or second primary tumor (SPT). In this study, we use TP53 mutation analysis for validation of an allelic loss marker panel and a decision algorithm for distinguishing between DM and SPT. Experimental Design: Tumor pairs of 39 patients were analyzed for TP53 mutations, for patterns of allelic loss and immunohistochemical analysis of p53 expression. Results of these three analyses were compared, using mutation analysis as gold standard. Results: Loss of heterozygosity (LOH) analysis indicated DM in 15 and SPT in 23 cases (one inconclusive). TP53 mutation analysis was informative in 15 cases. Based on the p53 mutation status alone, nine tumors were diagnosed as SPT and six as DM. In all 15 cases the LOH analysis was in concordance with the TP53 mutation analysis. Immunostaining for p53 showed promise as a first scan to diagnose lung tumors as SPT but cannot be used to diagnose DM. Conclusion: The TP53 mutation data validate the suitability of the LOH marker panel and decision algorithm for differential diagnosis of DM and SPT in the lung. LOH analysis can theoretically be exploited in almost all cases and is less laborious than TP53 mutation analysis. |
| Databáze: | OpenAIRE |
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