A Majority of Low (1-10%) ER Positive Breast Cancers Behave Like Hormone Receptor Negative Tumors
| Autor: | Aruna Korlimarla, Msn Prasad, Rohini Raghavan, Suraj Manjunath, C E Anupama, N Raman, Anjali Kalamdani, Nandini Dendukuri, Annie Alexander, Krisha Desai, Jyothi S. Prabhu, T. S. Sridhar, Kodaganur S. Gopinath, M Correa, B.S. Srinath |
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| Rok vydání: | 2014 |
| Předmět: |
Oncology
medicine.medical_specialty Pathology business.industry q-RT-PCR Estrogen receptor FFPE medicine.disease Subclass Staining ER 1-10 % Text mining Breast cancer Hormone receptor Internal medicine Breast Cancer Gene expression gene expression medicine Immunohistochemistry business Research Paper |
| Zdroj: | Journal of Cancer |
| ISSN: | 1837-9664 |
| Popis: | Background: The 2010 guidelines by ASCO-CAP have mandated that breast cancer specimens with ≥1% positively staining cells by immunohistochemistry should be considered Estrogen Receptor (ER) positive. This has led to a subclass of low-ER positive (1-10%) breast cancers. We have examined the biology and clinical behavior of these low ER staining tumors. Methods: We have developed a probabilistic score of the “ER-positivity” by quantitative estimation of ER related gene transcripts from FFPE specimens. Immunohistochemistry for ER was done on 240 surgically excised tumors of primary breast cancer. Relative transcript abundance of 3 house-keeping genes and 6 ER related genes were determined by q-RT PCR. A logistic regression model using 3 ER associated genes provided the best probability function, and a cut-off value was derived by ROC analysis. 144 high ER (>10%), 75 ER negative and 21 low-ER (1-10%) tumors were evaluated using the probability score and the disease specific survival was compared. Results: Half of the low-ER positive tumors were assigned to the ER negative group based on the probability score; in contrast 95% of ER negative and 92% of the high ER positive tumors were assigned to the appropriate ER group (p |
| Databáze: | OpenAIRE |
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