Modulation of Transforming Growth Factor-β Actions in Rat Osteoblast-like Cells: The Effects of bFGF and EGF
| Autor: | Stanley L. Chang, Joanne Bednarz Mallory, Theresa L. Chen |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
medicine.medical_specialty
TGF alpha Clinical Biochemistry Basic fibroblast growth factor In Vitro Techniques chemistry.chemical_compound Endocrinology Epidermal growth factor Internal medicine medicine Animals Growth factor receptor inhibitor Cells Cultured Osteoblasts Epidermal Growth Factor Chemistry Cell growth Rats Inbred Strains Osteoblast DNA Cell Biology Alkaline Phosphatase Fibronectins Rats Fibroblast Growth Factors medicine.anatomical_structure Transforming growth factor beta 3 Transforming Growth Factors Collagen Cell Division Transforming growth factor |
| Zdroj: | Growth Factors. 1:335-345 |
| ISSN: | 1029-2292 0897-7194 |
| DOI: | 10.3109/08977198909000257 |
| Popis: | The present study examines how the mitogenic and differentiation functions of transforming growth factor-beta (TGF-beta) are modulated by basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) in primary cultures of rat osteoblast-like (ROB) cells. TGF-beta, bFGF, and EGF individually stimulated [3H]thymidine incorporation and cell proliferation in a dose range of 0.01-10 ng/ml. When studied in combination, high doses of bFGF and EGF were additive to low doses of TGF-beta. The additive effects of bFGF and EGF on mitogenesis diminished with increasing doses of TGF-beta. These three factors also decreased alkaline phosphatase activity individually within the same dose range. When cells were treated with the combined factors, only high doses of bFGF and EGF were additive to the TGF-beta inhibition. We were unable to detect any change in collagen synthesis with each individual factor or in combined treatments. In addition, TGF-beta or bFGF alone or in combination did not affect fibronectin synthesis. Our studies showed that the biological functions of TGF-beta can be modulated by bFGF and EGF in ROB cells. The pattern of modulation is varied depending on the specific function examined. |
| Databáze: | OpenAIRE |
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