Association of hemolytic activity of Pseudomonas entomophila, a versatile soil bacterium, with cyclic lipopeptide production

Autor: Isabelle Vallet-Gely, Bruno Lemaitre, Luis A. Augusto, Pierre Cosson, Maria Péchy-Tarr, Gérard Bolbach, Alexey Novikov, Peter Liehl, Martine Caroff, Christoph Keel
Přispěvatelé: Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Centre de génétique moléculaire (CGM), Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC), Département de Microbiologie Fondamentale, Université de Lausanne (UNIL), Département de Physiologie Cellulaire et Métabolisme, Université de Genève (UNIGE), Université de Lausanne = University of Lausanne (UNIL), Université de Genève = University of Geneva (UNIGE)
Rok vydání: 2010
Předmět:
RNA
Untranslated
MESH: Drosophila
Swarming motility
MESH: Amino Acid Sequence
MESH: Virulence
MESH: Genome
Bacterial
Applied Microbiology and Biotechnology
MESH: RNA
Untranslated
chemistry.chemical_compound
MESH: Lipopeptides
Peptides
Cyclic/*biosynthesis/chemistry/metabolism
MESH: Endopeptidase Clp
RNA
Untranslated/genetics/metabolism
MESH: Pest Control
Biological
MESH: Animals
MESH: Endopeptidases
MESH: Bacterial Proteins
Soil Microbiology
Repressor Proteins/genetics/metabolism
chemistry.chemical_classification
0303 health sciences
MESH: Gene Expression Regulation
Bacterial
Ecology
biology
Virulence
Hemolytic Agents
Pseudomonas
Lipopeptide
Endopeptidase Clp
Pseudomonas putida
RNA
Bacterial
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Biochemistry
MESH: Repressor Proteins
Pseudomonadales
Bacterial Proteins/genetics/metabolism
Drosophila
MESH: Genes
Bacterial
MESH: RNA
Bacterial
Pseudomonas entomophila
RNA
Bacterial/genetics/metabolism
Biotechnology
Virulence Factors
Peptides
Cyclic
Lipopeptides/*biosynthesis/chemistry/metabolism
Virulence Factors/genetics/metabolism
Microbiology
Virulence/genetics
03 medical and health sciences
Lipopeptides
Bacterial Proteins
Nonribosomal peptide
Endopeptidases
Invertebrate Microbiology
Animals
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Amino Acid Sequence
ddc:612
Pest Control
Biological
Drosophila/genetics/metabolism
MESH: Peptides
Cyclic
Endopeptidases/genetics/metabolism
030304 developmental biology
MESH: Virulence Factors
030306 microbiology
MESH: Pseudomonas
Gene Expression Regulation
Bacterial
Hemolytic Agents/chemistry/*metabolism
biology.organism_classification
Repressor Proteins
chemistry
MESH: Soil Microbiology
Genes
Bacterial
Pseudomonas/*genetics/metabolism/pathogenicity
Endopeptidase Clp/genetics/metabolism
MESH: Hemolytic Agents
Genome
Bacterial
Food Science
Zdroj: Applied and environmental microbiology
Applied and Environmental Microbiology
Applied and Environmental Microbiology, American Society for Microbiology, 2010, 76 (3), pp.910-21. ⟨10.1128/AEM.02112-09⟩
Applied and Environmental Microbiology, Vol. 76, No 3 (2010) pp. 910-921
Applied and Environmental Microbiology, 2010, 76 (3), pp.910-21. ⟨10.1128/AEM.02112-09⟩
ISSN: 0099-2240
1098-5336
DOI: 10.1128/AEM.02112-09
Popis: Pseudomonas entomophila is an entomopathogenic bacterium that is able to infect and kill Drosophila melanogaster upon ingestion. Its genome sequence suggests that it is a versatile soil bacterium closely related to Pseudomonas putida . The GacS/GacA two-component system plays a key role in P. entomophila pathogenicity, controlling many putative virulence factors and AprA, a secreted protease important to escape the fly immune response. P. entomophila secretes a strong diffusible hemolytic activity. Here, we showed that this activity is linked to the production of a new cyclic lipopeptide containing 14 amino acids and a 3-C 10 OH fatty acid that we called entolysin. Three nonribosomal peptide synthetases (EtlA, EtlB, EtlC) were identified as responsible for entolysin biosynthesis. Two additional components (EtlR, MacAB) are necessary for its production and secretion. The P. entomophila GacS/GacA two-component system regulates entolysin production, and we demonstrated that its functioning requires two small RNAs and two RsmA-like proteins. Finally, entolysin is required for swarming motility, as described for other lipopeptides, but it does not participate in the virulence of P. entomophila for Drosophila . While investigating the physiological role of entolysin, we also uncovered new phenotypes associated with P. entomophila , including strong biocontrol abilities.
Databáze: OpenAIRE
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