15-Hydroxy-eicosatetraenoic acid (15-Hete) inhibits carragheenan-induced experimental arthritis and reduces synovial fluid leukotriene B4 (LTB4)
| Autor: | K. Fogh, E. Stender Hansen, T. Herlin, V. Knudsen, T. Brink Henriksen, H. Ewald, C. Bünger, K. Kragballe |
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| Jazyk: | angličtina |
| Rok vydání: | 1989 |
| Předmět: |
medicine.medical_specialty
Neutrophils Leukotriene B4 Eicosatetraenoic acid Radioimmunoassay Chemokinesis Arthritis Carrageenan Biochemistry Dinoprostone Proinflammatory cytokine chemistry.chemical_compound Dogs Endocrinology Internal medicine Hydroxyeicosatetraenoic Acids Synovial Fluid medicine Animals Synovial fluid Chromatography High Pressure Liquid medicine.disease Chemotaxis Leukocyte chemistry lipids (amino acids peptides and proteins) Arachidonic acid |
| Zdroj: | Fogh, K, Stender Hansen, E, Herlin, T, Knudsen, V, Brink Henriksen, T, Ewald, H, Bünger, C & Kragballe, K 1989, ' 15-Hydroxy-eicosatetraenoic acid (15-Hete) inhibits carragheenan-induced experimental arthritis and reduces synovial fluid leukotriene B 4 (LTB 4 ) ', Prostaglandins, vol. 37, no. 2, pp. 213-228 . https://doi.org/10.1016/0090-6980(89)90058-0 |
| DOI: | 10.1016/0090-6980(89)90058-0 |
| Popis: | 15-Hydroxy-eicosatetraenoic acid (15-HETE), a product of arachidonic acid, has no proinflammatory capacity, but can inhibit the formation and the chemotactic response of neutrophils to leukotriene B4 (LTB4), a potent mediator of inflammation. The purpose of the present study was to determine whether intraarticular administration of 15-HETE in carragheenan-induced acute arthritis might decrease the levels of LTB4 in synovial fluid and modify the arthritis. A bilateral acute knee joint arthritis was established in 7 dogs by intraarticular injections of carragheenan every third day. To the right joints, 15-HETE was administered both concomitantly with the carragheenan injections and continously via an osmotic pump. In samples of synovial fluid obtained on day 0, 3 and 10 PGE2 and LTB4 were determined using reversed phase high performance liquid chromatography combined with radioimmunoassays and neutrophil chemokinesis. In the presence of 15-HETE the clinical severity of arthritis was significantly reduced and the volume synovial effusate was decreased on an average by 42%. Furthermore, the relative number of neutrophils in histological sections of synovial tissue was decreased by 58%. Intaarticular carragheenan injection induced LTB4 formation, and maximum levels were obtained on day 3 (279.2 ± 148.2 pg/joint). PGE2 was also present on a day 3, but maximum levels were detected on day 10 (9.5 ± 4.8 ng/joint). In joints injected with both carragheenan and 15-HETE the levels of LTB4 on days 3 and 10 were inhibited by 90% and 83%, respectively. For PGE2 a small but significant decrease was found on both day 3 and on day 10. These results show that LTB4 may be an important mediator of acute arthritis induced by carragheenan in dogs, and that intraarticular administration of 15- HETE can modify this arthritis by inhibiting LTB4 formation. |
| Databáze: | OpenAIRE |
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