Ex vivo real-time monitoring of volatile metabolites resulting from nasal odorant metabolism
| Autor: | Philippe Faure, Aline Robert-Hazotte, Jean-Marie Heydel, Jean-Luc Le Quéré, Etienne Sémon, Loïc Briand, Elisabeth Guichard, Rachel Schoumacker |
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| Přispěvatelé: | Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), Université Bourgogne Franche-Comté [COMUE] (UBFC), The Agence Nationale de la Recherche (Qualisgraphy), FEDER grant (fonds européen de développement régional), Regional Council of Burgundy grant (plan d’actions régional pour l’innovation), The Ministry of Higher education and Research., Heydel, Jean-Marie |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Chimie-Physique lcsh:Medicine Olfaction Article 03 medical and health sciences Olfactory mucosa 0302 clinical medicine medicine Food and Nutrition Volatile metabolites lcsh:Science chemistry.chemical_classification Multidisciplinary Chemical Physics musculoskeletal neural and ocular physiology lcsh:R Metabolism 030104 developmental biology Enzyme medicine.anatomical_structure Biochemistry chemistry Alimentation et Nutrition lcsh:Q [PHYS.PHYS.PHYS-CHEM-PH]Physics [physics]/Physics [physics]/Chemical Physics [physics.chem-ph] Gas chromatography–mass spectrometry [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition 030217 neurology & neurosurgery Ex vivo Function (biology) psychological phenomena and processes |
| Zdroj: | Scientific Reports Scientific Reports, Nature Publishing Group, 2019, 9 (1), pp.2492. ⟨10.1038/s41598-019-39404-x⟩ Scientific Reports, Vol 9, Iss 1, Pp 1-13 (2019) Scientific Reports 1 (9), 2492. (2019) |
| ISSN: | 2045-2322 |
| Popis: | Odorant-metabolizing enzymes are critically involved in the clearance of odorant molecules from the environment of the nasal neuro-olfactory tissue to maintain the sensitivity of olfactory detection. Odorant metabolism may also generate metabolites in situ, the characterization and function of which in olfaction remain largely unknown. Here, we engineered and validated an ex vivo method to measure odorant metabolism in real-time. Glassware containing an explant of rat olfactory mucosa was continuously flushed with an odorant flow and was coupled to a proton transfer reaction-mass spectrometer for volatile compound analysis. Focusing on carboxylic esters and diketone odorants, we recorded the metabolic uptake of odorants by the mucosa, concomitantly with the release of volatile odorant metabolites in the headspace. These results significantly change the picture of real-time in situ odorant metabolism and represent a new step forward in the investigation of the function of odorant metabolites in the peripheral olfactory process. Our method allows the systematic identification of odorant metabolites using a validated animal model and permits the screening of olfactory endogenously produced chemosensory molecules. |
| Databáze: | OpenAIRE |
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