The role of the transforming A gene of SV40 in the mutagenic activity of the virus
| Autor: | Marshak Mi, Varshaver Nb, Gorbunova Lv, N.I. Shapiro |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Chromosome Aberrations
biology Genes Viral Mercaptopurine Mutant Drug Resistance Simian virus 40 Gene mutation biology.organism_classification Cell Transformation Viral Chromosome aberration Molecular biology Chinese hamster Virus Cell Line Cricetulus Antigen Cricetinae Mutation Genetics Animals Permissive Molecular Biology Gene |
| Zdroj: | Moleculargeneral genetics : MGG. 187(3) |
| ISSN: | 0026-8925 |
| Popis: | The mutagenic activity of the tsA239 mutant of SV40 which synthetizes a defective T antigen at 40 degrees C was investigated in Chinese hamster cells under permissive and nonpermissive temperature. At 33 degrees C the virus increased the yield of 6-mercaptopurine-resistant colonies after 2 days expression time by a factor of 1.6-4 as compared with the control and raised the frequency of aberrant metaphases after the same time by a factor of 1.9-3.4. In the same experiments, with the same initially infected population of Chinese hamster cells, at 40 degrees C tsA SV40 did not induce either gene mutations or chromosome aberrations at the same early stage after infection. Presumably the activity of the A gene of SV40 is necessary not only for the transforming but also for the mutagenic effect of the virus. |
| Databáze: | OpenAIRE |
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