Molecular genetics of chimpanzee Rh-related genes: Their relationship with the R-C-E-F blood group system, the chimpanzee counterpart of the human rhesus system
| Autor: | J. Clayton, A. Blancher, I. Salvignol, P. Calvas, J. Ruffie, W. W. Socha, Yves Colin |
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| Rok vydání: | 1994 |
| Předmět: |
Pan troglodytes
Molecular Sequence Data Polymerase Chain Reaction Biochemistry Restriction fragment chemistry.chemical_compound Sequence Homology Nucleic Acid Genetics Animals Molecular Biology Gene Ecology Evolution Behavior and Systematics Southern blot Base Sequence biology Haplotype Nucleic acid sequence General Medicine Molecular biology Pedigree genomic DNA chemistry Blood Group Antigens biology.protein Sequence Alignment Rh blood group system DNA |
| Zdroj: | Biochemical Genetics. 32:201-221 |
| ISSN: | 1573-4927 0006-2928 |
| Popis: | As the chimpanzee R-C-E-F blood group system appears to be the chimpanzee counterpart of the human Rhesus (RH) system, we have tried to determine whether chimpanzee Rh-like genes encode R-C-E-F-related proteins. Chimpanzee genomic DNA, digested by any of eight endonucleases and hybridized with three Rh exon-specific probes, exhibits a high degree of polymorphism. Analysis of DNA from unrelated individuals of different R-C-E-F types revealed that the presence of some restriction fragments is correlated with particular R-C-E-F types. The cosegregation of these fragments with R-C-E-F haplotypes was confirmed by family studies. Oligonucleotides complementary to regions flanking human exons were used as PCR primers on chimpanzee DNA; the resulting amplified fragments were identical in size to their human counterparts. Moreover, the nucleotide sequences of the fragments present a high degree of similarity to the corresponding human regions. |
| Databáze: | OpenAIRE |
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