Transcript profiling of expressed sequence tags from semimembranosus muscle of commercial and naturalized pig breeds
| Autor: | C. F. Campos, M. E. Botelho, M.M.D.C.A. Weller, Maysa Machado, Jane de Oliveira Peixoto, C.S. Nascimento, V.R. Faria, F. F. e Silva, L.L. Verardo, Marta Fonseca Martins, S.E.F. Guimarães, Paulo Sávio Lopes |
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| Přispěvatelé: | CARLOS SOUZA DO NASCIMENTO, UFV, JANE DE OLIVEIRA PEIXOTO, CNPSA, UFV, MAYARA MORENA DEL CAMBRE AMARAL WELLER, UFV, MARTA FONSECA MARTINS, CNPGL, MARCO ANTONIO MACHADO, CNPGL, SIMONE ELISA FACIONI GUIMARAES, UFV. |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
DNA
Complementary Molecular Sequence Data Sus scrofa Breeding Biology cDNA library Homology (biology) Semimembranosus muscle Qualidade da carne Myosin Genetics Presunto Animals Genomic library RNA Messenger Databases Protein Muscle Skeletal Suíno Molecular Biology Gene Library Expressed Sequence Tags Expressed sequence tag Gene Expression Profiling Commerce Proteins Molecular Sequence Annotation Sequence Analysis DNA General Medicine Anatomy DNA Genética Breed Ham Gene expression profiling Gene Expression Regulation Expressed sequence tags Organ Specificity Muscle |
| Zdroj: | LOCUS Repositório Institucional da UFV Universidade Federal de Viçosa (UFV) instacron:UFV Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA Scopus-Elsevier |
| Popis: | In general, genetic differences across different breeds of pig lead to variation in mature body size and slaughter age. The Commercial breeds Duroc and Large White and the local Brazilian breed Piau are ostensibly distinct in terms of growth and muscularity, commercial breeds are much leaner while local breeds grow much slower and are fat type pigs. However, the genetic factors that underlie such distinctions remain unclear. We used expressed sequence tags (ESTs) to characterize and compare transcript profiles in the semimembranosus muscle of these pig breeds. Our aim was to identify differences in breed-related gene expression that might influence growth performance and meat quality. We constructed three non-normalized cDNA libraries from semimembranosus muscle, using two samples from each one, of these three breeds; 6902 high-quality ESTs were obtained. Cluster analysis was performed and these sequences were clustered into 3670 unique sequences; 24.7% of the sequences were categorized as contigs and 75.3% of the sequences were singletons. Based on homology searches against the SwissProt protein database, we were able to assign a putative protein identity to only 1050 unique sequences. Among these, 58.5% were full-length protein sequences and 17.2% were pig-specific sequences. Muscle structural and cytoskeletal proteins, such as actin, and myosin, were the most abundant transcripts (16.7%) followed by those related to mitochondrial function (12.9%), and ribosomal proteins (12.4%). Furthermore, ESTs generated in this study provide a rich source for identification of novel genes and for the comparative analysis of gene expression patterns in divergent pig breeds. |
| Databáze: | OpenAIRE |
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