A programmable platform for sub-second multichemical dynamic stimulation and neuronal functional imaging in C. elegans
| Autor: | Guillaume Aubry, Yongmin Cho, Tel Rouse, Manuel Zimmer, Hang Lu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Sensory Receptor Cells Computer science Cytological Techniques Biomedical Engineering Bioengineering Stimulation Stimulus (physiology) Cellular level Biochemistry Article 03 medical and health sciences Lab-On-A-Chip Devices Premovement neuronal activity Animals Caenorhabditis elegans Cells Cultured biology General Chemistry Equipment Design Microfluidic Analytical Techniques biology.organism_classification Stimulation Chemical Functional imaging 030104 developmental biology Neuronal circuits Neuroscience |
| Zdroj: | Lab on a chip. 18(3) |
| ISSN: | 1473-0189 |
| Popis: | Caenorhabditis elegans (C. elegans) is a prominent model organism in neuroscience, as its small stereotyped nervous system offers unique advantages for studying neuronal circuits at the cellular level. Characterizing temporal dynamics of neuronal circuits is essential to fully understand neuronal processing. Characterization of the temporal dynamics of chemosensory circuits requires a precise and fast method to deliver multiple stimuli and monitor the animal's neuronal activity. Microfluidic platforms have been developed that offer an improved control of chemical delivery compared to manual methods. However, stimulating an animal with multiple chemicals at high speed is still difficult. In this work, we have developed a platform that can deliver any sequence of multiple chemical reagents, at sub-second resolution and without cross-contamination. We designed a network of chemical selectors wherein the chemical selected for stimulation is determined by the set of pressures applied to the chemical reservoirs. Modulation of inlet pressures has been automated to create robust, programmable sequences of subsecond chemical pulses. We showed that stimulation with sequences of different chemicals at the second to sub-second range can generate different neuronal activity patterns in chemosensory neurons; we observed previously unseen neuronal responses to a controlled chemical stimulation. Because of the speed and versatility of stimulus generated, this platform opens new possibilities to investigate neuronal circuits. |
| Databáze: | OpenAIRE |
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