Repurposing of escitalopram oxalate and clonazepam in combination with ciprofloxacin and sulfamethoxazole–trimethoprim for treatment of multidrug-resistant microorganisms and evaluation of the cleavage capacity of plasmid DNA
| Autor: | Vitória Segabinazzi Foletto, Catrine de Souza Machado, Vinícius Victor Lorenzoni, Angelita Bottega, Rosmari Hörner, Tacieli Fagundes da Rosa, Roberta Filipini Rampelotto, Marissa Bolson Serafin, Amanda Mainardi, Silvana Silveira Coelho |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Sulfamethoxazole medicine.drug_class 030106 microbiology Immunology Antibiotics Microbial Sensitivity Tests Citalopram Pharmacology Gram-Positive Bacteria Applied Microbiology and Biotechnology Microbiology Clonazepam Trimethoprim 03 medical and health sciences chemistry.chemical_compound Minimum inhibitory concentration Antibiotic resistance Ciprofloxacin Drug Resistance Multiple Bacterial Gram-Negative Bacteria Genetics medicine Humans Sulfamethoxazole/Trimethoprim Molecular Biology Minimum bactericidal concentration Drug Repositioning DNA General Medicine Escitalopram Oxalate Anti-Bacterial Agents Multiple drug resistance 030104 developmental biology chemistry Plasmids medicine.drug |
| Zdroj: | Canadian Journal of Microbiology. 67:599-612 |
| ISSN: | 1480-3275 0008-4166 |
| Popis: | Bacterial resistance has become one of the most serious public health problems, globally, and drug repurposing is being investigated to speed up the identification of effective drugs. The aim of this study was to investigate the repurposing of escitalopram oxalate and clonazepam drugs individually, and in combination with the antibiotics ciprofloxacin and sulfamethoxazole–trimethoprim, to treat multidrug-resistant (MDR) microorganisms and to evaluate the potential chemical nuclease activity. The minimum inhibitory concentration, minimum bactericidal concentration, fractional inhibitory concentration index, and tolerance level were determined for each microorganism tested. In vitro antibacterial activity was evaluated against 47 multidrug-resistant clinical isolates and 11 standard bacterial strains from the American Type Culture Collection. Escitalopram oxalate was mainly active against Gram-positive bacteria, and clonazepam was active against both Gram-positive and Gram-negative bacteria. When associated with the two antibiotics mentioned, they had a significant synergistic effect. Clonazepam cleaved plasmid DNA, and the mechanisms involved were oxidative and hydrolytic. These results indicate the potential for repurposing these non-antibiotic drugs to treat bacterial infections. However, further studies on the mechanism of action of these drugs should be performed to ensure their safe use. |
| Databáze: | OpenAIRE |
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