Effects of combined inositol hexakisphosphate and inositol supplement on antioxidant activity and metabolic enzymes in the liver of streptozotocin-induced type 2 diabetic rats
| Autor: | Ruby L. Alexander-Lindo, Shadae R. Foster, Lowell Dilworth, Felix O. Omoruyi, Rory Thompson |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Antioxidant Phytic Acid Normal diet medicine.medical_treatment Glucosephosphate Dehydrogenase Toxicology Antioxidants Streptozocin Diabetes Mellitus Experimental Rats Sprague-Dawley Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Diabetes mellitus medicine Animals Inositol Triglycerides biology Chemistry Liver cell Bilirubin General Medicine medicine.disease Streptozotocin Glutathione Rats Up-Regulation Enzyme Activation Oxidative Stress 030104 developmental biology Endocrinology Liver 030220 oncology & carcinogenesis Dietary Supplements biology.protein Alkaline phosphatase Lipoproteins HDL Oxidoreductases medicine.drug |
| Zdroj: | Chemico-Biological Interactions. 275:108-115 |
| ISSN: | 0009-2797 |
| DOI: | 10.1016/j.cbi.2017.07.024 |
| Popis: | Diabetes mellitus is associated with elevated reactive oxygen species, lipid abnormalities, reduced antioxidant activity and organ damage. This study examines the effects of combined inositol hexakisphosphate (IP6) and inositol supplement on antioxidant levels and other biochemical parameters in the liver of type 2 diabetic rats. Five groups of Sprague-Dawley rats were studied. Six rats were fed normal diet (non-diabetic control), while 24 rats were fed high-fat diet (HFD) for 4 weeks. Diabetes was induced in 18 of the rats fed HFD by intraperitoneal administration of streptozotocin. The diabetic rats were separated into three groups namely: combined IP6 and inositol, glibenclamide and diabetic control. The non-diabetic group fed high-fat diet was classified as a high-fat control group. For the final four weeks of the experiment, all rats were fed normal diet and given their respective treatment regimes. Hepatic antioxidant status, metabolic enzyme activity, lipid profile, peroxidative damage and liver histology, as well as, serum aminotransferase and alkaline phosphatase activities, and total bilirubin concentration were assessed. Treatment with combined IP6 and inositol supplement significantly increased liver reduced glutathione and high-density lipoprotein levels while liver triglyceride levels and serum alkaline phosphatase activity were significantly reduced by 27%, 50%, 38.5%, and 69.2% respectively compared to the diabetic control. Hepatic superoxide dismutase, catalase, glucose-6-phosphate dehydrogenase activities were significantly upregulated by 55%, 26% and 53% respectively in the diabetic rats treated with combined IP6 and inositol compared to the diabetic control. Combined IP6 and inositol treatment resulted in the preservation of liver cell integrity and improved antioxidant status in type 2 diabetic rats. |
| Databáze: | OpenAIRE |
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