Additive effect of oestradiol-17 and serum on synthesis of deoxyribonucleic acid in guinea-pig endometrial cells in culture
| Autor: | M. Alkhalaf, Gérard L. Adessi, A. Mahfoudi, A. Y. Propper |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Embryology
medicine.medical_specialty medicine.medical_treatment Guinea Pigs Stimulation Biology Endometrium Endocrinology Epidermal growth factor Internal medicine medicine Animals Insulin Beta (finance) Cells Cultured Fetus Epidermal Growth Factor Estradiol DNA synthesis Growth factor Obstetrics and Gynecology Drug Synergism DNA Cell Biology Culture Media Reproductive Medicine Cell culture Female Thymidine |
| Zdroj: | Reproduction. 93:295-302 |
| ISSN: | 1741-7899 1470-1626 |
| DOI: | 10.1530/jrf.0.0930295 |
| Popis: | Normal guinea-pig endometrial cells, grown in primary culture, were made quiescent by serum depletion. Quiescent cells cultured in the control medium (containing 1% fetal calf serum treated with dextran-coated charcoal, DCC-FCS) showed a steady and weak rate of [3H]thymidine incorporation, but the addition of 15% fetal calf serum (FCS) or 10% DCC-FCS to the control medium induced a significant increase of DNA synthesis, demonstrating the responsiveness of the quiescent cells to stimulation. A lower but significant increase in [3H]thymidine incorporation was elicited by epidermal growth factor (EGF, 100 ng/ml) or insulin (10 micrograms/ml) added to the basal medium. Oestradiol-17 beta added to the control medium at concentrations ranging from 10(-10) to 10(-5) mol/l not only failed to increase but even inhibited [3H]thymidine incorporation at the highest concentrations tested. An additive effect was noticed when quiescent cells were incubated with oestradiol-17 beta (10(-9) mol/l) in the presence of 10% DCC-FCS, but no synergistic effect occurred when 2 x 10(-9) mol oestradiol-17 beta/l was combined with either EGF (100 ng/ml) or insulin (10 micrograms/ml). Oestradiol-17 beta appears unable alone to stimulate DNA synthesis in normal endometrial cells, but requires factor(s) present in fetal calf serum. |
| Databáze: | OpenAIRE |
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