Imaging in vivo acetylcholine release in the peripheral nervous system with a fluorescent nanosensor
| Autor: | Nicholas Micovic, Junfei Xia, Hongrong Yang, James R. Monaghan, Heather A. Clark, Michelle Mu, Kira E. Poskanzer |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Stimulation Bioengineering Biosensing Techniques Neurotransmission Inbred C57BL Efferent nerve Cholinergic Antagonists chemistry.chemical_compound Mice In vivo Receptors submandibular ganglion medicine Animals Nanotechnology Receptors Cholinergic Cholinergic Fluorescent Dyes Multidisciplinary Neurosciences Parasympathetic Ganglia Parasympathetic DNA acetylcholinesterase Submandibular ganglion Carbocyanines Hydrogen-Ion Concentration Bungarotoxins Acetylcholinesterase Acetylcholine acetylcholine Mice Inbred C57BL medicine.anatomical_structure chemistry Peripheral nervous system Physical Sciences Neurological Biophysics Ganglia Female nanosensor medicine.drug |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America, vol 118, iss 14 Proc Natl Acad Sci U S A |
| Popis: | The ability to monitor the release of neurotransmitters during synaptic transmission would significantly impact the diagnosis and treatment of neurological diseases. Here, we present a DNA-based enzymatic nanosensor for quantitative detection of acetylcholine (ACh) in the peripheral nervous system of living mice. ACh nanosensors consist of DNA as a scaffold, acetylcholinesterase as a recognition component, pH-sensitive fluorophores as signal generators, and α-bungarotoxin as a targeting moiety. We demonstrate the utility of the nanosensors in the submandibular ganglia of living mice to sensitively detect ACh ranging from 0.228 to 358 μM. In addition, the sensor response upon electrical stimulation of the efferent nerve is dose dependent, reversible, and we observe a reduction of ∼76% in sensor signal upon pharmacological inhibition of ACh release. Equipped with an advanced imaging processing tool, we further spatially resolve ACh signal propagation on the tissue level. Our platform enables sensitive measurement and mapping of ACh transmission in the peripheral nervous system. |
| Databáze: | OpenAIRE |
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