Cloning and functional characterisation of two novel nicotinic acetylcholine receptor alpha subunits from the insect pest Myzus persicae
| Autor: | F. Sgard, M. J. Katkowska, S. J. Dunbar, Mustafa B.A. Djamgoz, Scott P. Fraser, J. D. Windass |
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| Rok vydání: | 1998 |
| Předmět: |
DNA
Complementary Protein subunit Xenopus Molecular Sequence Data Biology Receptors Nicotinic Biochemistry Cellular and Molecular Neuroscience Botany medicine Animals Drosophila Proteins Amino Acid Sequence Cloning Molecular Acetylcholine receptor G alpha subunit Sequence Homology Amino Acid fungi Cell biology Nicotinic acetylcholine receptor Nicotinic agonist Aphids Oocytes Female Drosophila Protein Acetylcholine Cys-loop receptors medicine.drug |
| Zdroj: | Journal of neurochemistry. 71(3) |
| ISSN: | 0022-3042 |
| Popis: | Nicotinic acetylcholine receptors play a major role in excitatory neurotransmission in insect CNSs and constitute an important target for insecticides. Here, we report the isolation and functional characterisation of two cDNAs encoding nicotinic acetylcholine receptor alpha subunits from a major insect pest, the peach-potato aphid Myzus persicae. These two subunits, termed Mp alpha1 and Mp alpha2, are respective structural homologues of the Drosophila D alpha2/Schistocerca gregaria alphaL1 alpha-subunit pair and the Drosophila ALS alpha subunit. Xenopus oocyte expression confirmed that each Myzus subunit can form functional acetylcholine- or nicotine-gated channels. However, some electrophysiological and pharmacological properties of the Myzus subunits were distinct from those encoded by the corresponding Drosophila subunits. Coexpression of the Myzus subunits with the chick beta2 subunit revealed other differences from the Drosophila system, as only very limited potentiation of agonist-induced currents was observed with Mp alpha2 and none with Mp alpha1. Available data therefore indicate that structurally homologous insect nicotinic acetylcholine receptor alpha subunits from different species can exhibit distinctive physiological and pharmacological characteristics. |
| Databáze: | OpenAIRE |
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