Genetic differences between two Leishmania major-like strains revealed by suppression subtractive hybridization
| Autor: | Soraia de Oliveira Silva, Jorge L. Pesquero, João Bosco Pesquero, Michelle A. R. Freitas, Maria Norma Melo, Maria Carmen Aires Gomes, Ângela C.A. Wu, Paula Molina Nogueira, Rodrigo Pedro Soares |
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| Rok vydání: | 2015 |
| Předmět: |
Subtractive Hybridization Techniques
DNA Complementary Protozoan Proteins Virulence Leishmaniasis Cutaneous Glycosphingolipids chemistry.chemical_compound Mice Complementary DNA Cricetinae Sequence Homology Nucleic Acid Animals Humans Leishmania major Molecular Biology Gene Genetics Mice Inbred BALB C biology Mesocricetus Macrophages Neglected Diseases Lipophosphoglycan DNA Protozoan Leishmania biology.organism_classification Galactosyltransferases Mice Inbred C57BL chemistry Suppression subtractive hybridization Parasitology |
| Zdroj: | Molecular and biochemical parasitology. 203(1-2) |
| ISSN: | 1872-9428 |
| Popis: | Leishmania major, the causative agent of zoonotic leishmaniasis, is restricted to Old World countries. Molecular and biochemical techniques have been used to identify some L. major-like isolated in South America including Brazil. Here, two L. major-like strains, one virulent (BH49) and one non-virulent (BH121), were subjected to suppression subtractive hybridization (SSH) technique in order to identify differentially expressed genes. SSH technique identified nine cDNA fragments exhibiting high homology to previously sequenced L. major genes. Five cDNAs (four specific for BH49 and one for BH121) were confirmed by RT-PCR. Among those differentially expressed subtracted genes, some were involved in physiological processes including metabolism, translation and destination of proteins, production of energy, virulence factors and unknown functions. Western-blot analysis confirmed a higher expression level of β-1,3-galactosyl residues in L. major-like lipophosphoglycan (LPG). This molecular analysis opens the possibility for identification of potential virulence factors not only in different strains, but also in others species of Leishmania. |
| Databáze: | OpenAIRE |
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