Expression of auxilin or AP180 inhibits endocytosis by mislocalizing clathrin: evidence for formation of nascent pits containing AP1 or AP2 but not clathrin
| Autor: | Xiaohong Zhao, Hadi Al-Hasani, Samuel W. Cushman, Evan Eisenberg, Tsvika Greener, Lois E. Greene |
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| Rok vydání: | 2001 |
| Předmět: |
Monosaccharide Transport Proteins
Adaptor Protein Complex 1 Adaptor Protein Complex 2 Muscle Proteins Nerve Tissue Proteins Auxilin Biology Endocytosis Transfection Clathrin Tensins Chlorocebus aethiops Animals Humans HSP70 Heat-Shock Proteins Sequence Deletion Glucose Transporter Type 4 Vesicle Microfilament Proteins HSC70 Heat-Shock Proteins Transferrin Biological Transport Coated Pits Cell-Membrane Cell Biology Phosphoproteins In vitro Recombinant Proteins Cell biology AP-1 transcription factor Adaptor Proteins Vesicular Transport Kinetics Monomeric Clathrin Assembly Proteins COS Cells biology.protein Ap180 Clathrin adaptor proteins HeLa Cells |
| Zdroj: | Journal of cell science. 114(Pt 2) |
| ISSN: | 0021-9533 |
| Popis: | Although uncoating of clathrin-coated vesicles is a key event in clathrin-mediated endocytosis it is unclear what prevents uncoating of clathrin-coated pits before they pinch off to become clathrin-coated vesicles. We have shown that the J-domain proteins auxilin and GAK are required for uncoating by Hsc70 in vitro. In the present study, we expressed auxilin in cultured cells to determine if this would block endocytosis by causing premature uncoating of clathrin-coated pits. We found that expression of auxilin indeed inhibited endocytosis. However, expression of auxilin with its J-domain mutated so that it no longer interacted with Hsc70 also inhibited endocytosis as did expression of the clathrin-assembly protein, AP180, or its clathrin-binding domain. Accompanying this inhibition, we observed a marked decrease in clathrin associated with the plasma membrane and the trans-Golgi network, which provided us with an opportunity to determine whether the absence of clathrin from clathrin-coated pits affected the distribution of the clathrin assembly proteins AP1 and AP2. Surprisingly we found almost no change in the association of AP2 and AP1 with the plasma membrane and the trans-Golgi network, respectively. This was particularly obvious when auxilin or GAK was expressed with functional J-domains since, in these cases, almost all of the clathrin was sequestered in granules that also contained Hsc70 and auxilin or GAK. We conclude that expression of clathrin-binding proteins inhibits clathrin-mediated endocytosis by sequestering clathrin so that it is no longer available to bind to nascent pits but that assembly proteins bind to these pits independently of clathrin. |
| Databáze: | OpenAIRE |
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