Electrophoretic variants of blood proteins in Japanese
| Autor: | Takeshi Kageoka, Norio Takahashi, Ryuji Hazama, Chiyoko Satoh, Jun-ichi Asakawa, Kazuaki Goriki, Junko Kaneko, Mikio Fujita |
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| Rok vydání: | 1984 |
| Předmět: |
Male
Erythrocytes Population Triosephosphate isomerase Gene Frequency parasitic diseases Genetics medicine Humans Isoelectric Point education Genetics (clinical) Gel electrophoresis education.field_of_study Polymorphism Genetic biology Isoelectric focusing Blood proteins Molecular biology Enzyme assay Pedigree Red blood cell Starch gel electrophoresis medicine.anatomical_structure biology.protein Female Carbohydrate Epimerases Triose-Phosphate Isomerase |
| Zdroj: | Human Genetics. 68:185-188 |
| ISSN: | 1432-1203 0340-6717 |
| Popis: | A total of 15,387 individuals living in Hiroshima and Nagasaki, of whom 10,864 are unrelated, were examined for erythrocyte triosephosphate isomerase (TPI) by starch gel electrophoresis using TEMM buffer, pH 7.4. Four kinds of new variants, one having a cathodal migration and three having anodal migrations, were encountered in this population. These variants were further characterized by starch gel electrophoresis using tris-EDTA buffer, pH 9.3, and isoelectric focusing. An anodally migrating allozyme TPI 2HR1 exhibited markedly decreased enzyme activity, as evaluated by the staining intensity of the variant bands. The level of TPI activity in erythrocytes from this individual with the phenotype TPI 1-2HR1 was about 60% of the normal mean. Family studies confirmed the genetic nature of all the variants. |
| Databáze: | OpenAIRE |
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