Potential intake of vitamins 'A' and 'D' through branded intravenous lipid emulsions: Liquid Chromatography-Tandem Mass Spectrometry Analysis

Autor: Rita Mancini, Daniela Patrono, Andrea Pession, Germana Bersani, Matteo Conti, Maria Luisa Forchielli, C. Puggioli
Přispěvatelé: Forchielli, Maria Luisa, Conti, Matteo, Patrono, Daniela, Mancini, Rita, Pession, Andrea, Puggioli, Cristina, Bersani, Germana
Rok vydání: 2015
Předmět:
0301 basic medicine
Vitamin
Fat Emulsions
Intravenous
food.ingredient
Liquid Chromatography-Tandem Mass Spectrometry Analysi
Critical Care and Intensive Care Medicine
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
food
Fish Oils
Liquid chromatography–mass spectrometry
Tandem Mass Spectrometry
Branded intravenous lipid emulsion
Nutrition and Dietetic
Medicine
Vitamin D
Vitamin A
Alkaline hydrolysis
Olive Oil
Triglycerides
030109 nutrition & dietetics
Nutrition and Dietetics
Chromatography
Retinol (vitamin A) and ergo-chole-calciferol (vitamin D2/D3)
business.industry
010401 analytical chemistry
Coconut oil
Body Weight
Retinol
Fish oil
0104 chemical sciences
Soybean Oil
Hexane
Fat-Soluble Vitamin
chemistry
Biochemistry
Coconut Oil
business
Chromatography
Liquid
Zdroj: Clinical nutrition (Edinburgh, Scotland). 36(2)
ISSN: 1532-1983
Popis: Summary No data exist for vitamin A group and vitamin D2/D3 content in branded intravenous lipid emulsions (ILEs). Our goal is to evaluate and quantify their concentrations in different ILEs to assess whether they are clinically relevant. Analyses were carried out in triplicates on six ILEs: 1) 30% soybean oil-based, 2) 20% olive-soybean oil based, 3) 10 + 10% soybean - MCT coconut oil based, 4) 20% soybean-olive-MCT-fish oil based, 5) 20% soybean-MCT-fish oil based and 6) 10% pure fish oil based, respectively. Retinol group (vitamin A) and ergo-chole-calciferol (vitamin D2/D3) were analyzed and quantified by a quali-quantitative Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) method after potassium hydroxide alkaline hydrolysis, hexane extraction, reverse phase-liquid chromatography and specific multiple-reaction-monitoring (MRM) detection. On average, measured retinol content was in the range of 200–1000 μg/L in ILEs (1,2, and 3), whereas it was higher (1000–2000 μg/L) in the ILEs containing fish-oil. Vitamin D content was in the range of 1–10 μg/L in the fish-oil based ILEs, but undetectable in those ILEs containing purely vegetable oils. This study shows that vitamin A and D contents are variably present in ILEs based on their different lipid sources. Both contents should be explicitly mentioned in the products.
Databáze: OpenAIRE
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