Laryngeal Mucosa Alterations in Mice Model of Gastroesophageal Reflux: Effects of Topical Protection
| Autor: | Lorena Brito da Silva, Suliana Mesquita Paula, Lucas A.D. Nicolau, Maria Klayre A. Sousa, Flávio Maria Nobre Othon Sidou, André Alencar Araripe Nunes, Daniel Sifrim, Renata Rocha Nascimento, Humberto Barbosa da Costa-Filho, Durcilene Alves da Silva, Rhubens Levy R. Moreira, Thiago M. Sales, Marcellus H.L.P. Souza, Aline A. Figueiredo, João P. Carmo-Neto, Jand V.R. Medeiros |
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| Rok vydání: | 2019 |
| Předmět: |
Larynx
Male medicine.medical_specialty Alginates Inflammation Gastroenterology Laryngeal Mucosa 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Laryngitis Internal medicine Hyaluronic acid Plant Gums Medicine Animals Anacardium Fluorescein Hyaluronic Acid 030223 otorhinolaryngology Barrier function biology business.industry Reflux Disease Models Animal medicine.anatomical_structure Otorhinolaryngology chemistry Myeloperoxidase biology.protein Gastroesophageal Reflux 030211 gastroenterology & hepatology medicine.symptom business |
| Zdroj: | The LaryngoscopeBIBLIOGRAPHY. 130(12) |
| ISSN: | 1531-4995 |
| Popis: | Objectives/hypothesis The objectives of this study were to evaluate laryngeal inflammation and mucosal integrity in a murine model of reflux disease and to assess the protective effects of topical agents including alginate, hyaluronic acid, and cashew gum. Study design Animal study. Methods A surgical murine model of reflux disease was evaluated at 3 or 7 days postsurgery, and laryngeal samples were collected to measure inflammation (wet weight and myeloperoxidase [MPO]) and mucosal integrity (transepithelial resistance [TER] and mucosal permeability to fluorescein). Additional groups of animals were administered one of several topical agents (alginate, hyaluronic acid, or cashew gum) daily, and laryngeal inflammation and mucosal integrity were evaluated at 3 days postsurgery. Results At 3 days, and not 7 days postsurgery, we observed increased laryngeal wet weight and MPO, decreased laryngeal TER, and increased laryngeal mucosa permeability. Alginate partially decreased laryngeal inflammation (wet weight and not MPO) and dramatically improved laryngeal mucosal integrity. Conversely, hyaluronic acid eliminated the inflammation; however, it had no effect on laryngeal mucosal integrity impairment. Cashew gum eliminated laryngeal inflammation as well as the impairment in laryngeal mucosal integrity. Conclusions This study shows that a surgical model of reflux disease induced laryngeal inflammation and impairment in laryngeal barrier function. These observed alterations were partially attenuated by alginate and hyaluronic acid and completely reversed by cashew gum. Level of evidence NA Laryngoscope, 2020. |
| Databáze: | OpenAIRE |
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