Aberrant development of motor axons and neuromuscular synapses in erbB2-deficient mice
| Autor: | Hugo B. Sanchez, Jacqueline K. Morris, T. J. Deerinck, Mark H. Ellisman, Kuo-Fen Lee, Weichun Lin |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
animal structures
Receptor ErbB-2 Diaphragm Neuromuscular Junction Muscle Proteins Intercostal Muscles Nerve Tissue Proteins Neuromuscular junction Receptor tyrosine kinase Synapse Embryonic and Fetal Development Mice Mice Neurologic Mutants Perisynaptic schwann cells Cell Movement Postsynaptic potential Morphogenesis medicine Animals Myocyte Receptors Cholinergic skin and connective tissue diseases neoplasms In Situ Hybridization Fluorescence Neuregulins Acetylcholine receptor Mice Knockout Motor Neurons Multidisciplinary biology fungi Gene Expression Regulation Developmental Biological Sciences Genes erbB-2 Axons Cell biology Phrenic Nerve medicine.anatomical_structure nervous system Nerve Degeneration biology.protein Neuregulin Schwann Cells |
| Zdroj: | Proceedings of the National Academy of Sciences. 97:1299-1304 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.97.3.1299 |
| Popis: | Receptor tyrosine kinase erbB2, which is activated by neuregulin, is expressed in Schwann and muscle cells in the developing neuromuscular junction (NMJ). In vitro studies have shown that neuregulin promotes the survival and migration of Schwann cells and stimulates acetylcholine receptor gene transcription in cultured muscle cells. These findings suggest an important role for erbB2 in the development of the NMJ. Here we examine erbB2-deficient mice to determine whether erbB2 is required for NMJ development in vivo . Our analysis shows that there are pre- and postsynaptic defects of developing NMJ in erbB2-deficient embryos. The presynaptic defects include defasciculation and degeneration of the motor nerves, and an absence of Schwann cells. The postsynaptic defect features an impairment of junctional folds at the neuromuscular synapse in the mutants. These results demonstrate that erbB2 is essential for in vivo development of the NMJ. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|