Transcriptome genetic differences between responders and non-responders before bronchial thermoplasty
| Autor: | Yukio Ishii, Yuzuru Kondo, Michiko Masuda, Masashi Matsuyama, Masayuki Nakajima, Satoshi Ano, Norihiro Kikuchi, Hajime Osawa, Nobuyuki Hizawa |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Pulmonary and Respiratory Medicine
medicine.medical_specialty Bronchi macromolecular substances Disease Gastroenterology Transcriptome Internal medicine medicine Immunology and Allergy Humans Asthma Bronchial Thermoplasty Bronchial thermoplasty business.industry Proteins Airway smooth muscle medicine.disease respiratory tract diseases Non responders Pediatrics Perinatology and Child Health Quality of Life RNA Histopathology Refractory asthma business |
| Zdroj: | The Journal of asthma : official journal of the Association for the Care of Asthma. 59(8) |
| ISSN: | 1532-4303 |
| Popis: | Bronchial thermoplasty (BT) is an endoscopic therapy used for the treatment of refractory asthma. Some predictive factors, for example the number of activations and severity of disease at baseline, have been used to determine the effectiveness of BT in treating patients with asthma. The aim of the present study was to comprehensively analyze RNA samples from the airway bronchial tissues of patients with severe asthma treated by BT, and to characterize each patient as a BT responder or non-responder.Eight patients with severe asthma scheduled to undergo BT and bronchus biopsies were recruited before the procedures were conducted. Extracted RNA samples from bronchial tissues were sequenced and differential gene expression analysis was carried out.Results/discussion: Subjects with Asthma Quality of Life Questionnaire score changes ≥0.5 for a period of 12 months were considered BT responders. Non-responders had score changes0.5 for 12 months. Histopathology findings were similar to those reported previously, and no significant differences in the expression of α-smooth muscle actin and protein gene product 9.5 were observed between responders and non-responders. Transcriptome analysis at baseline identified 67 genes that were differentially expressed between responders and non-responders, including |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|