A transgenic approach for controlling Lygus in cotton
| Autor: | Anilkumar Gowda, Robert S. Brown, Timothy J. Rydel, Wollacott Andrew M, Xiaohong Shi, Thomas L. Clark, Stanislaw Flasinski, Nageotte Jeffrey R, Brent J. Werner, Waseem Akbar, Michael Pleau, James A. Baum, Andrew C. Read |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Multidisciplinary biology business.industry Transgene Science Heteroptera fungi Pest control General Physics and Astronomy food and beverages General Chemistry Gossypium biology.organism_classification General Biochemistry Genetics and Molecular Biology Article Genetically modified organism 03 medical and health sciences 030104 developmental biology Agronomy Bacillus thuringiensis Bioassay Lygus business |
| Zdroj: | Nature Communications Nature Communications, Vol 7, Iss 1, Pp 1-7 (2016) |
| ISSN: | 2041-1723 |
| Popis: | Lygus species of plant-feeding insects have emerged as economically important pests of cotton in the United States. These species are not controlled by commercial Bacillus thuringiensis (Bt) cotton varieties resulting in economic losses and increased application of insecticide. Previously, a Bt crystal protein (Cry51Aa2) was reported with insecticidal activity against Lygus spp. However, transgenic cotton plants expressing this protein did not exhibit effective protection from Lygus feeding damage. Here we employ various optimization strategies, informed in part by protein crystallography and modelling, to identify limited amino-acid substitutions in Cry51Aa2 that increase insecticidal activity towards Lygus spp. by >200-fold. Transgenic cotton expressing the variant protein, Cry51Aa2.834_16, reduce populations of Lygus spp. up to 30-fold in whole-plant caged field trials. One transgenic event, designated MON88702, has been selected for further development of cotton varieties that could potentially reduce or eliminate insecticide application for control of Lygus and the associated environmental impacts. Plant-feeding insects of the Lygus genus have emerged as a major pest effecting cotton crops in the USA. Here the authors optimize the insecticidal activity of a Bacillus thuringiensis crystal protein and produce transgenic plants that are resistant to feeding damage by Lygus species. |
| Databáze: | OpenAIRE |
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