Potential Use of MALDI-ToF Mass Spectrometry for Rapid Detection of Antifungal Resistance in the Human Pathogen Candida glabrata
| Autor: | Antonietta Vella, Elena De Carolis, Dominique Sanglard, Enrica Mello, Brunella Posteraro, David S. Perlin, Maurizio Sanguinetti |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Antifungal Agents Anidulafungin/pharmacology Anidulafungin/therapeutic use Antifungal Agents/pharmacology Antifungal Agents/therapeutic use Candida glabrata/drug effects Candida glabrata/genetics Candida glabrata/growth & development Candidiasis Invasive/drug therapy Candidiasis Invasive/microbiology Drug Resistance Fungal Fluconazole/pharmacology Fluconazole/therapeutic use Fungal Proteins/genetics Genetic Fitness Glucosyltransferases/genetics Humans Microbial Sensitivity Tests Microbial Viability/drug effects Mutation Phenotype Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization/methods Science 030106 microbiology Human pathogen Candida glabrata Drug resistance Anidulafungin Article Settore MED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICA Microbiology Fungal Proteins 03 medical and health sciences medicine Candidiasis Invasive Pathogen Fluconazole Fungal protein Microbial Viability Multidisciplinary biology biology.organism_classification bacterial infections and mycoses Glucosyltransferases Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Medicine Echinocandins medicine.drug |
| Zdroj: | Scientific reports, vol. 7, no. 1, pp. 9099 Scientific Reports, Vol 7, Iss 1, Pp 1-9 (2017) Scientific Reports |
| Popis: | The echinocandins are relatively new antifungal drugs that represent, together with the older azoles, the recommended and/or preferred agents to treat candidaemia and other forms of invasive candidiasis in human patients. If “time is of the essence” to reduce the mortality for these infections, the administration of appropriate antifungal therapy could be accelerated by the timely reporting of laboratory test results. In this study, we attempted to validate a MALDI-ToF mass spectrometry-based assay for the antifungal susceptibility testing (AFST) of the potentially multidrug-resistant pathogen Candida glabrata against anidulafungin and fluconazole. The practical applicability of the assay, reported here as MS-AFST, was assessed with a panel of clinical isolates that were selected to represent phenotypically and genotypically/molecularly susceptible or resistant strains. The data show the potential of our assay for rapid detection of antifungal resistance, although the MS-AFST assay performed at 3 h of the in vitro antifungal exposure failed to detect C. glabrata isolates with echinocandin resistance-associated FKS2 mutations. However, cell growth kinetics in the presence of anidulafungin revealed important cues about the in vitro fitness of C. glabrata isolates, which may lead to genotypic or phenotypic antifungal testing in clinical practice. |
| Databáze: | OpenAIRE |
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