Transduction of Surface and Basal Cells in Rhesus Macaque Lung Following Repeat Dosing with AAV1CFTR
| Autor: | Murali K. Yanda, Cristina V. Cebotaru, William B. Guggino, Liudmila Cebotaru |
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| Rok vydání: | 2020 |
| Předmět: |
Cystic Fibrosis
Genetic Vectors Cystic Fibrosis Transmembrane Conductance Regulator Spleen Genome Viral Cystic fibrosis Virus Green fluorescent protein 03 medical and health sciences 0302 clinical medicine Transduction Genetic Genetics medicine Animals Humans Molecular Biology Lung 030304 developmental biology 0303 health sciences biology Dose-Response Relationship Drug ELISPOT Gene Transfer Techniques Genetic Therapy respiratory system Dependovirus medicine.disease biology.organism_classification Molecular biology Macaca mulatta Cystic fibrosis transmembrane conductance regulator respiratory tract diseases Titer Rhesus macaque medicine.anatomical_structure 030220 oncology & carcinogenesis biology.protein Molecular Medicine Research Article |
| Zdroj: | Hum Gene Ther |
| ISSN: | 1557-7422 |
| Popis: | To test the effectiveness of repeat dosing, we sprayed two doses (10(13) vg each) of AAV1Δ27-264-CFTR into airways of four rhesus monkeys at 0 and 30 days, followed by a single dose of 10(13) vg of AAV1GFP on day 60. Monkeys were sacrificed on day 90. No adverse events occurred, indicating that AAV1 vectors are safe. An elevated anti-AAV1 neutralizing titer was established by the third dose. A positive ELISPOT to the adeno-associated virus (AAV) capsid but not to cystic fibrosis transmembrane conductance regulator (CFTR) occurred after the third dose in three monkeys. AAV1-CFTR and GFP vectors were detectable in all lung sections and in the heart, liver, and spleen. The CFTR protein was higher in treated monkeys than in an untreated monkey. GFP protein was detected in treated lungs. Lung surface and keratin 5-positive basal cells showed higher CFTR staining than in the uninfected monkey and were positive for GFP staining, indicating widespread gene transduction by AAV1CFTR and GFP. AAV1 safely and effectively transduces monkey airway and basal cells. Both the significant numbers of vector genomes and transduction from AAV1CFTR and GFP virus seen in the monkeys 3 months after the first instillation suggest that repeat dosing with AAV1-based vectors is achievable. |
| Databáze: | OpenAIRE |
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