Emulating human microcapillaries in a multi-organ-chip platform
| Autor: | Roland Lauster, Tobias Hasenberg, Severin Mühleder, Wolfgang Holnthoner, Krystyna Labuda, Andrea Dotzler, Uwe Marx, Heinz Redl, Sophie Bauer |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Stromal cell
Time Factors Green Fluorescent Proteins Microfluidics Cell Culture Techniques Bioengineering Applied Microbiology and Biotechnology Polymerase Chain Reaction Fibrin Tissue culture Vasculogenesis Tissue engineering Human Umbilical Vein Endothelial Cells Humans Tube formation biology Tissue Engineering Tissue Scaffolds Stem Cells General Medicine Coculture Techniques Cell biology Capillaries Culture Media Perfusion Adipose Tissue Fibrin scaffold Cell culture Immunology biology.protein Rheology Transcriptome Gels Biotechnology |
| Zdroj: | Journal of biotechnology. 216 |
| ISSN: | 1873-4863 |
| Popis: | Current microfluidic chip-based tissue culture systems lack a capillary endothelial vessel system, which would enable perfusion with blood. We utilise spatial cell cultures to populate a perfused multi-organ-chip platform-a microfluidic device recently introduced for substance testing. Complete biological vascularization of such culture systems is vital to properly emulate physiological tissue behaviour. In this study, we incorporated a fibrin scaffold into the two-organ-chip design. Herein, adipose-derived stromal cells (ASCs) directed human umbilical vein endothelial cells (HUVECs) to organise into tube-like structures. The ASCs induced tube formation of HUVECs in static and dynamic conditions. The replacement of full medium enriched with growth factors and foetal calf serum with basal medium resulted in viable cells with similar gene expression profiles. We regard this as a prerequisite for studies with organ constructs that have a need for a different medium formulation. Furthermore, we here address stability issues of the fibrin gel and fibrin composition for optimal microvessel formation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect