Serological testing for Bartonella henselae infections in The Netherlands: clinical evaluation of immunofluorescence assay and ELISA
| Autor: | P. J. van Dijken, Harold Verbakel, M. Herremans, A.M.C. Bergmans, Marcel F. Peeters, Marijn J. Vermeulen, J. J. Roord |
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| Rok vydání: | 2007 |
| Předmět: |
Microbiology (medical)
Adult Adolescent diagnosis Enzyme-Linked Immunosorbent Assay Immunofluorescence Sensitivity and Specificity law.invention Serology law Bartonella henselae Infections Genotype medicine Humans immunofluorescence assay Child Fluorescent Antibody Technique Indirect Polymerase chain reaction Aged Netherlands Aged 80 and over Bartonella henselae biology medicine.diagnostic_test Cat-Scratch Disease Infant Cat-scratch disease General Medicine Middle Aged biology.organism_classification medicine.disease Virology Antibodies Bacterial Infectious Diseases PCR Immunoglobulin M Child Preschool Immunoglobulin G Immunology biology.protein ELISA Antibody |
| Zdroj: | Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases. 13(6) |
| ISSN: | 1198-743X |
| Popis: | Cat-scratch disease (CSD), caused by Bartonella henselae infection, can mimic malignancy and can manifest atypically. Reliable serological testing is therefore of great clinical importance. The diagnostic performance of immunofluorescence assay (IFA) and ELISA was evaluated in a group of Dutch patients with proven CSD (clinical diagnosis confirmed by PCR). Sera of 51 CSD patients and 56 controls (patients with similar symptoms, but who were B. henselae PCR-negative and had an alternative confirmed diagnosis) were tested for anti-B. henselae IgM and IgG by IFA and ELISA. A commercially available IFA test for IgM had a sensitivity of 6%. In-house assays for IgM showed specificities of 93% (IFA) and 91% (ELISA), but with low sensitivities (53% and 65%, respectively). With a specificity of 82% (IFA) and 91% (ELISA), in-house IgG testing showed a significantly higher sensitivity in IFA (67%) than in ELISA (28%, p |
| Databáze: | OpenAIRE |
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