Copper Induces Vasorelaxation and Antagonizes Noradrenaline -Induced Vasoconstriction in Rat Mesenteric Artery
| Autor: | De-Li Dong, Rong Huo, Hong-Chao Jiang, Chao-Wei Hu, Yu-Chun Wang, Ming-Yu Liu |
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| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Physiology chemistry.chemical_element Blood Pressure In Vitro Techniques Nitric Oxide lcsh:Physiology Nitric oxide lcsh:Biochemistry Rats Sprague-Dawley Norepinephrine chemistry.chemical_compound In vivo Internal medicine medicine Animals Vasoconstrictor Agents lcsh:QD415-436 Mesenteric arteries Chelating Agents lcsh:QP1-981 Chemistry Vasorelaxation Copper Mesenteric Arteries Rats Vasodilation NG-Nitroarginine Methyl Ester Endocrinology medicine.anatomical_structure Blood pressure Vasoconstriction Anesthesia Potassium Rabbits Hypotension medicine.symptom Ditiocarb Myograph Artery |
| Zdroj: | Cellular Physiology and Biochemistry, Vol 32, Iss 5, Pp 1247-1254 (2013) |
| ISSN: | 1421-9778 1015-8987 |
| Popis: | Background/Aims: Copper is an essential trace element for normal cellular function and contributes to critical physiological or pathological processes. The aim of the study was to investigate the effects of copper on vascular tone of rat mesenteric artery and compare the effects of copper on noradrenaline (NA) and high K+ induced vasoconstriction. Methods: The rat mesenteric arteries were isolated and the vessel tone was measured by using multi wire myograph system in vitro. Blood pressure of carotid artery in rabbits was measured by using physiological data acquisition and analysis system in vivo. Results: Copper dose-dependently blunted NA-induced vasoconstriction of rat mesenteric artery. Copper-induced vasorelaxation was inhibited when the vessels were pretreated with NG-nitro-L-arginine methyl ester (L-NAME). Copper did not blunt high K+-induced vasoconstriction. Copper preincubation inhibited NA-evoked vasoconstriction and the inhibition was not affected by the presence of L-NAME. Copper preincubation showed no effect on high K+-evoked vasoconstriction. Copper chelator diethyldithiocarbamate trihydrate (DTC) antagonized the vasoactivity induced by copper in rat mesenteric artery. In vivo experiments showed that copper injection (iv) significantly decreased blood pressure of rabbits and NA or DTC injection (iv) did not rescue the copper-induced hypotension and animal death. Conclusion: Copper blunted NA but not high K+-induced vasoconstriction of rat mesenteric artery. The acute effect of copper on NA-induced vasoconstriction was depended on nitric oxide (NO), but the effect of copper pretreatment on NA-induced vasoconstriction was independed on NO, suggesting that copper affected NA-induced vasoconstriction by two distinct mechanisms. |
| Databáze: | OpenAIRE |
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