Flow cytometric analysis of goat milk lymphocytes: subpopulations and adhesion molecule expression
| Autor: | Timothy Greenland, Jean François Mornex, François Guiguen, Geneviève Panaye, Eliane Pardo |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
General Veterinary
biology medicine.diagnostic_test medicine.drug_class Goats Immunology Integrin food and beverages Alpha (ethology) Monoclonal antibody Flow Cytometry Molecular biology Lymphocyte Subsets Staining Flow cytometry Beta-1 adrenergic receptor Milk biology.protein medicine Animals L-selectin Cell Adhesion Molecules CD8 |
| Zdroj: | Veterinary immunology and immunopathology. 53(1-2) |
| ISSN: | 0165-2427 |
| Popis: | Cytometric analysis of cells in milk from healthy goats was achieved after elimination of interfering signals from debris and dead cells by irreversible staining with ethidium monoazide. Some 61% of milk lymphocytes are CD8+ T cells, 17% are CD4+ and about 20% of these express class II antigens: less than 4% are B cells. Compared with blood, the CD4/CD8 ratio was inverted and fewer lymphocytes expressed CD4SR or L-selectin. Monoclonal antibodies to ovine integrins recognised the caprine alpha 4 chain on most lymphocytes from milk or blood, while beta 1 was more frequent on milk cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect