A comparative study of protein patterns of human estrogen receptor positive (MCF-7) and negative (MDA-MB-231) breast cancer cell lines
| Autor: | Markéta Laštovičková, Julius Brtko, Dana Macejova, Lucia Toporova, Dana Flodrová, Janette Bobalova |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Proteome Physiology Biophysics Estrogen receptor Breast Neoplasms Vimentin 03 medical and health sciences 0302 clinical medicine Breast cancer Biomarkers Tumor medicine Humans skin and connective tissue diseases Receptor biology General Medicine medicine.disease Molecular biology Neoplasm Proteins Gene Expression Regulation Neoplastic 030104 developmental biology Receptors Estrogen MCF-7 030220 oncology & carcinogenesis MCF-7 Cells biology.protein Annexin A2 Annexin A1 |
| Zdroj: | General physiology and biophysics. 35:387-392 |
| ISSN: | 1338-4325 |
| Popis: | In the present study, we analyzed the cell lysates of human tumour cell lines representing two major clinically different types of breast cancer. Our main goal was to show the differences between them on proteomic level. Gel electrophoresis followed by MALDI-TOF MS analysis was used for proteins determination. Exactly 98 proteins were unequivocally identified and 60 of them were expressed differentially between MDA-MB-231 and MCF-7 cell lines. Among the proteins reported here, some well-known breast cancer markers (e.g., annexin A1, annexin A2 and vimentin) were identified in the MDA-MB-231 cell line and thus we were able to distinguish both cell lines sufficiently. |
| Databáze: | OpenAIRE |
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