Droplet microfluidics platform for highly sensitive and quantitative detection of malaria-causing Plasmodium parasites based on enzyme activity measurement
| Autor: | Charlotte Harmsen, Rikke Frøhlich, Paola Fiorani, Birgitta R. Knudsen, Alessandro Desideri, Sissel Juul, Emil L. Kristoffersen, Rodrigo Labouriau, Eskild Petersen, Yi-Ping Ho, Cinzia Tesauro, Stéphane Picot, Pia W. Jensen, Kam W. Leong, Anne-Lise Bienvenu, Amit Roy, Jørn Koch, Magnus Stougaard, David Tordrup, Christine J. F. Nielsen, Ya-Ling Chiu, Janet Cox-Singh |
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| Přispěvatelé: | Duke University [Durham], Department of Molecular Biology, University of Aarhus, Università degli Studi di Roma Tor Vergata [Roma], Institute of Translational Pharmacology - Istituto di Farmacologia Traslazionale [Roma] (IFT), Consiglio Nazionale delle Ricerche [Roma] (CNR), University of Saint Andrews, University Malaysia Sarawak, Department of Pathology - Aarhus University, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS), University Hospital Skejby, Interdisciplinary Nanoscience Center (iNANO), Aarhus University [Aarhus], Depierre, Frédérique |
| Rok vydání: | 2012 |
| Předmět: |
Falciparum
diagnosis Plasmodium falciparum malaria General Physics and Astronomy 02 engineering and technology Plasmodium Article 03 medical and health sciences Species Specificity parasitic diseases medicine Humans General Materials Science Droplet microfluidics Malaria Falciparum 030304 developmental biology Plasmodium species Enzyme Assays Detection limit 0303 health sciences droplet microfluidics biology lab-on-a-chip Base Sequence Settore BIO/11 enzyme activity detection [CHIM.ORGA]Chemical Sciences/Organic chemistry General Engineering Microfluidic Analytical Techniques [CHIM.ORGA] Chemical Sciences/Organic chemistry 021001 nanoscience & nanotechnology biology.organism_classification medicine.disease Virology Molecular biology Enzyme assay rolling-circle amplification 3. Good health Highly sensitive biology.protein 0210 nano-technology Malaria |
| Zdroj: | ACS Nano ACS Nano, American Chemical Society, 2012, 6 (12), pp.10676-10683 Juul, S, Nielsen, C J F, Labouriau, R, Roy, A, Tesauro, C, Jensen, P W, Harmsen, C, Kristoffersen, E L, Chiu, Y-L, Hougaard, R F, Fiorani, P, Cox-Singh, J, Tordrup, D P, Koch, J E, Bienvenu, A-L, Desideri, A, Picot, S, Petersen, E, Leong, K W, Ho, Y-P, Stougaard, M & Knudsen, B R 2012, ' Droplet Microfluidics Platform for Highly Sensitive and Quantitative Detection of Malaria-Causing Plasmodium Parasites Based on Enzyme Activity Measurement ', A C S Nano, vol. 6, no. 12, pp. 10676-10683 . https://doi.org/10.1021/nn3038594 ACS nano 6 (2012): 10676–10683. doi:10.1021/nn3038594 info:cnr-pdr/source/autori:Juul S.; Nielsen C.J.F.; Labouriau R.; Roy A.; Tesauro C.; Jensen P.W.; Harmsen C.; Kristoffersen E.L.; Chiu Y.-L.; Frohlich R.; Fiorani P.; Cox-Singh J.; Tordrup D.; Koch J.; Bienvenu A.-L.; Desideri A.; Picot S.; Petersen E.; Leong K.W.; Ho Y.-P.; Stougaard M.; Knudsen B.R./titolo:Droplet microfluidics platform for highly sensitive and quantitative detection of malaria-causing plasmodium parasites based on enzyme activity measurement/doi:10.1021%2Fnn3038594/rivista:ACS nano/anno:2012/pagina_da:10676/pagina_a:10683/intervallo_pagine:10676–10683/volume:6 |
| ISSN: | 1936-086X 1936-0851 |
| Popis: | We present an attractive new system for the specific and sensitive detection of the malaria-causing Plasmodium parasites. The system relies on isothermal conversion of single DNA cleavage-ligation events catalyzed specifically by the Plasmodium enzyme topoisomerase I to micrometer-sized products detectable at the single-molecule level. Combined with a droplet microfluidics lab-on-a-chip platform, this design allowed for sensitive, specific, and quantitative detection of all human-malaria-causing Plasmodium species in single drops of unprocessed blood with a detection limit of less than one parasite/?L. Moreover, the setup allowed for detection of Plasmodium parasites in noninvasive saliva samples from infected patients. During recent years malaria transmission has declined worldwide, and with this the number of patients with low-parasite density has increased. Consequently, the need for accurate detection of even a few parasites is becoming increasingly important for the continued combat against the disease. We believe that the presented droplet microfluidics platform, which has a high potential for adaptation to point-of-care setups suitable for low-resource settings, may contribute significantly to meet this demand. Moreover, potential future adaptation of the presented setup for the detection of other microorganisms may form the basis for the development of a more generic platform for diagnosis, fresh water or food quality control, or other purposes within applied or basic science. © 2012 American Chemical Society. |
| Databáze: | OpenAIRE |
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