Evaluation of four laboratory-based SARS-CoV-2 IgG antibody immunoassays
| Autor: | Deborah De Geyter, Johan Schiettecatte, Ilse Weets, Maria Bjerke, Jorg Tanis, Ellen Vancutsem, Denis Pierard |
|---|---|
| Přispěvatelé: | Faculty of Medicine and Pharmacy, Microbiology and Infection Control, Clinical Biology, Supporting clinical sciences, Pharmaceutical and Pharmacological Sciences, Diabetes Pathology & Therapy, Clinical sciences, Movement and Sport Sciences, Basic (bio-) Medical Sciences, Experimental Pharmacology |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Adult Male medicine.medical_specialty 2019-20 coronavirus outbreak Epstein-Barr Virus Infections Coronavirus disease 2019 (COVID-19) Adolescent IgG Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) specificity Antibodies Viral Gastroenterology Sensitivity and Specificity COVID-19 Serological Testing Young Adult Sensitivity Internal medicine medicine Humans immunoassay Igg elisa Child skin and connective tissue diseases Aged Retrospective Studies biology medicine.diagnostic_test business.industry SARS-CoV-2 fungi Infant COVID-19 General Medicine Middle Aged body regions Infectious Diseases Immunoassay Child Preschool Immunoglobulin G biology.protein Female Original Article Antibody business |
| Zdroj: | Diagnostic Microbiology and Infectious Disease |
| ISSN: | 1879-0070 0732-8893 |
| Popis: | Objectives To evaluate the diagnostic performances of four SARS-CoV-2 IgG antibody immunoassays. Methods Following immunoassays were studied: Abbott's SARS-CoV-2 IgG assay, Diasorin's Liaison SARS-CoV-2 S2/S2 IgG assay, Euroimmun's Anti-SARS-CoV-2 IgG ELISA, and Roche's Elecsys Anti-SARS-CoV-2 assay. Specificity was retrospectively evaluated with 38 samples from 2019. Sensitivity samples (n = 147) were taken from SARS-CoV-2 real-time PCR-positive patients who developed COVID-19 symptoms ten days earlier. Results Mean specificity was 96.6%. Mean sensitivity was 62.7% from ten days after onset of symptoms, 84.4% from 15 days after onset of symptoms, and 87.5% from 20 days after onset of symptoms. Conclusions Specificity was high, while Abbott and Roche were 100% specific. Sensitivity increased over time, with Abbott and Roche having the highest sensitivity at all time points with ≥90% from 20 days after symptoms' onset. These findings may assist in selecting SARS-CoV-2 IgG antibody immunoassays for additional diagnostics, epidemiological research, and vaccine development. |
| Databáze: | OpenAIRE |
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