BC4707 Is a Major Facilitator Superfamily Multidrug Resistance Transport Protein from Bacillus cereus Implicated in Fluoroquinolone Tolerance

Autor: Aniko Vörös, Jaakko Ekman, Ingerid Nes, Peter J. F. Henderson, Kim E. Bettaney, Jasmin K. Kroeger, Mirja Salkinoja-Salonen, Roger Simm, Anne-Brit Kolstø, Marianne Södring, Massoud Saidijam
Přispěvatelé: Department of Food and Nutrition
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Transcription
Genetic
Bacillus cereus
lcsh:Medicine
bc4707
Antiport Proteins
medicine.disease_cause
Biochemistry
Microbial Physiology
lcsh:Science
Oligonucleotide Array Sequence Analysis
0303 health sciences
Multidisciplinary
biology
CCCP
Genomics
pTTQ18
Drug Resistance
Multiple
Bacterial Pathogens
Anti-Bacterial Agents
3. Good health
Transport protein
Protein Transport
Cereus
Cytochemistry
Efflux
microarray
Research Article
Fluoroquinolones
fluoroquinolone
Microbiology
Bile Acids and Salts
03 medical and health sciences
Bacterial Proteins
Stress
Physiological
multidrug resistance
Microbial Control
Genetics
medicine
Gene Silencing
Biology
Escherichia coli
030304 developmental biology
Gram Positive
030306 microbiology
Cell Membrane
lcsh:R
fungi
pBC4707
Membrane Proteins
Proteins
biology.organism_classification
Major facilitator superfamily
ATCC14579
Multiple drug resistance
bacteria
1182 Biochemistry
cell and molecular biology
lcsh:Q
Gene Function
Genome Expression Analysis
Bacteria
proton gradient
Zdroj: PLoS ONE; Vol 7
PLoS ONE, Vol 7, Iss 5, p e36720 (2012)
PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0036720
Popis: Transcriptional profiling highlighted a subset of genes encoding putative multidrug transporters in the pathogen Bacillus cereus that were up-regulated during stress produced by bile salts. One of these multidrug transporters (BC4707) was selected for investigation. Functional characterization of the BC4707 protein in Escherichia coli revealed a role in the energized efflux of xenobiotics. Phenotypic analyses after inactivation of the gene bc4707 in Bacillus cereus ATCC14579 suggested a more specific, but modest role in the efflux of norfloxacin. In addition to this, transcriptional analyses showed that BC4707 is also expressed during growth of B. cereus under non-stressful conditions where it may have a role in the normal physiology of the bacteria. Altogether, the results indicate that bc4707, which is part of the core genome of the B. cereus group of bacteria, encodes a multidrug resistance efflux protein that is likely involved in maintaining intracellular homeostasis during growth of the bacteria.
Databáze: OpenAIRE
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