Effect of vitamin C on azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced colitis-associated early colon cancer in mice
| Autor: | Yiseul Yeom, Yoo Sun Kim, Pu Reum Seok, Moon Jea Woo, Hee Jin Jeon, Yuri Kim, Eunju Kim, Jae Ho Shin |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
colonic neoplasm medicine.medical_specialty Colorectal cancer colitis medicine.medical_treatment Inflammation 03 medical and health sciences chemistry.chemical_compound Internal medicine medicine microbiota Vitamin C Colitis Original Research Nutrition and Dietetics Azoxymethane business.industry medicine.disease 030104 developmental biology Endocrinology chemistry inflammation Tumor necrosis factor alpha medicine.symptom business Dysbiosis Food Science Prostaglandin E |
| Zdroj: | Nutrition Research and Practice |
| ISSN: | 1976-1457 |
| Popis: | Background/objectives The objective of this study was to investigate the effects of vitamin C on inflammation, tumor development, and dysbiosis of intestinal microbiota in an azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced inflammation-associated early colon cancer mouse model. Materials/methods Male BALB/c mice were injected intraperitoneally with AOM [10 mg/kg body weight (b.w)] and given two 7-d cycles of 2% DSS drinking water with a 14 d inter-cycle interval. Vitamin C (60 mg/kg b.w. and 120 mg/kg b.w.) was supplemented by gavage for 5 weeks starting 2 d after the AOM injection. Results The vitamin C treatment suppressed inflammatory morbidity, as reflected by disease activity index (DAI) in recovery phase and inhibited shortening of the colon, and reduced histological damage. In addition, vitamin C supplementation suppressed mRNA levels of pro-inflammatory mediators and cytokines, including cyclooxygenase-2, microsomal prostaglandin E synthase-2, tumor necrosis factor-α, Interleukin (IL)-1β, and IL-6, and reduced expression of the proliferation marker, proliferating cell nuclear antigen, compared to observations of AOM/DSS animals. Although the microbial composition did not differ significantly between the groups, administration of vitamin C improved the level of inflammation-related Lactococcus and JQ084893 to control levels. Conclusion Vitamin C treatment provided moderate suppression of inflammation, proliferation, and certain inflammation-related dysbiosis in a murine model of colitis associated-early colon cancer. These findings support that vitamin C supplementation can benefit colonic health. Long-term clinical studies with various doses of vitamin C are warranted. |
| Databáze: | OpenAIRE |
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