MicroRNA-196b promotes gastric cancer progression by targeting ECRG4
| Autor: | Huirong Tang, Xi Cheng, Yong Zhu, Shufeng Xiao, Fujia Xie, Haibo Luo, Daoming Liang, Junlin Chi, Guobin Liu, Jun Ma, Yanlong Yang, Liao Chen, Xuesong Wu |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Male Cancer Research Down-Regulation Biology Real-Time Polymerase Chain Reaction 03 medical and health sciences Mice 0302 clinical medicine Downregulation and upregulation In vivo Cell Movement Stomach Neoplasms Cell Line Tumor microRNA medicine Animals Humans Pharmacology (medical) Aged Cell Proliferation Pharmacology Gene knockdown Cell growth Tumor Suppressor Proteins Cancer Cell migration Middle Aged medicine.disease Up-Regulation Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology Oncology 030220 oncology & carcinogenesis Cancer research Immunohistochemistry Female |
| Zdroj: | Anti-cancer drugs. 32(2) |
| ISSN: | 1473-5741 |
| Popis: | Gastric cancer is one of the most common malignant tumors. MicroRNA-196b (miR-196b) has been demonstrated to play important roles in human cancers. However, its functions in gastric cancer progression were still largely unknown. In this study, the expression of miR-196b was determined by quantitative real-time PCR. Esophageal cancer-related gene 4 (ECRG4) level was examined by western blot assay and immunohistochemistry staining assay. Cell proliferation was evaluated by Cell Counting Kit-8 (CCK-8) assay and colony formation assay. Cell migration and invasion were analyzed by transwell assay. The association between miR-196b and ECRG4 was analyzed by dual-luciferase reporter assay. The functional role of miR-196b in vivo was analyzed by murine xenograft assay. As a result, we found the expression of miR-196b was elevated and the protein expression of ECRG4 was reduced in gastric cancer tissues and cells. MiR-196b inhibition suppressed gastric cancer cell proliferation, migration and invasion. ECRG4 was a target of miR-196b and its protein expression was negatively regulated by miR-196b. Moreover, ECRG4 overexpression showed similar effects with miR-196b inhibition on the malignant behaviors of GC cells and ECRG4 knockdown reversed the effects of miR-196b inhibition on gastric cancer cell proliferation, migration and invasion. In addition, miR-196b inhibition suppressed tumor volume and weight in vivo. In conclusion, downregulation of miR-196b inhibited gastric cancer progression by modulating ECRG4 expression, indicating that miR-196b might be a potential therapeutic target for gastric cancer. |
| Databáze: | OpenAIRE |
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