Circulating miR-181a and miR-223 expression with the potential value of biomarkers for the diagnosis of systemic lupus erythematosus and predicting lupus nephritis
| Autor: | Nearmeen M. Rashad, Haidy E. Zidan, Walid S.H. Elsayed, Rehab S. Abdul-Maksoud, Nafesa M. Kamal, Manal A Ali |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Adult Male Population Lupus nephritis law.invention 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine mir-223 immune system diseases law Drug Discovery microRNA Genetics medicine Humans Lupus Erythematosus Systemic skin and connective tissue diseases education Molecular Biology Genetics (clinical) Polymerase chain reaction education.field_of_study Creatinine Proteinuria business.industry Curve analysis medicine.disease Prognosis Lupus Nephritis MicroRNAs 030104 developmental biology chemistry Gene Expression Regulation 030220 oncology & carcinogenesis Immunology Molecular Medicine Egypt Female medicine.symptom business Biomarkers |
| Zdroj: | The journal of gene medicineREFERENCES. 23(5) |
| ISSN: | 1521-2254 |
| Popis: | Background MicroRNAs (miRNAs) contribute to the development and progression of systemic lupus erythematosus (SLE) by affecting a wide range of targeted genes and facilitating the development of lupus nephritis (LN). The present study aimed to analyze the serum expression of miR-181a and miR-223 in SLE patients and to assess whether they could serve as novel biomarkers for SLE diagnosis and to distinguish LN. Methods The study included 70 control subjects and 116 patients with SLE (67 non-LN and 49 LN groups). Circulating miR-181a and miR-223 expression levels were analyzed among the Egyptian population using a real-time polymerase chain reaction. Results Up-regulation of miR-181a was detected among SLE patients compared to healthy controls and higher values were reported among the LN group compared to the non-LN group. Down-regulation of miR-223 was reported among SLE patients compared to controls and lower values were reported among the LN group compared to the non-LN group. The higher miR-181a expression and the lower miR-223 expression were associated with higher stages of LN. SLE disease activity index, proteinuria and serum creatinine were independently correlated with miR-181a and miR-223 among SLE patients by linear regression analysis. Receiver-operating characteristic curve analysis revealed that combined miR-181a and miR-223 expression increased the sensitivity and specificity for the diagnosis of SLE and further distinguished LN from non-LN patients. Conclusions miR-181a and miR-223 could play a role in evaluating SLE disease progression and prognosis. Combined miR-181a and miR-223 expression analysis could serve as novel serum-based biomarkers in the diagnosis of SLE and predicting LN among Egyptians. |
| Databáze: | OpenAIRE |
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