Anti-capsular antibodies activate killing of Escherichia coli O8:K87 by the alternate complement pathway in porcine serum
| Autor: | Emil M. Berberov, Rodney A. Moxley, M. Wang, N. M. Clark |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Swine
Immunology Complement Pathway Alternative Enzyme-Linked Immunosorbent Assay medicine.disease_cause Microbiology Mice Affinity chromatography Antigen Enterotoxigenic Escherichia coli medicine Escherichia coli Animals Bovine serum albumin Bacterial Capsules Escherichia coli Infections Swine Diseases Mice Inbred BALB C General Veterinary biology Immune Sera Antibodies Bacterial Complement system Specific Pathogen-Free Organisms biology.protein Alternative complement pathway Female Immunization Rabbits Antibody |
| Zdroj: | Veterinary immunology and immunopathology. 114(1-2) |
| ISSN: | 0165-2427 |
| Popis: | Enterotoxigenic Escherichia coli (ETEC) strains that produce K88 (F4)+ fimbria are important causes of diarrhea and post-diarrheal septicemia in swine. ETEC O8:K87, a serotype represented by a number of these strains, is typically serum resistant. Strain-specific antibodies are known to activate alternative C pathway-mediated killing of other serum-resistant E. coli [Hill, A.W., Shears, A.L., Hibbitt, K.G., 1978. The requirement of specific antibody for the killing of E. coli by the alternate complement pathway in bovine serum. Immunology 34, 131-136], but their antigenic targets have not been determined. We tested the hypothesis that anti-K87 antibodies activate alternative pathway-mediated killing of ETEC O8:K87. Pigs were immunized with ETEC O8:K87 strain 2534-86 cells or purified K87 polysaccharide. Post-, but not pre-immunization sera killed 2534-86 cells, and absorption with 2534-86 cells or by K87 affinity chromatography eliminated bactericidal activity. Complementation of absorbed serum with anti-K87 antibodies restored bactericidal activity, confirming the ability of these antibodies to activate C-mediated serum killing. Serum from age-matched, non-vaccinated control pigs also killed 2534-86. This activity was eliminated by absorption with 2534-86 cells, but not K87 affinity chromatography, indicating that specific non-capsular antibodies are also able to activate C-mediated killing. In all cases, Mg-EGTA-treated serum was as effective as non-treated serum in killing, suggesting that bactericidal activity was mediated predominantly if not exclusively via the alternative C pathway. |
| Databáze: | OpenAIRE |
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