Coding RNA Sequencing of Equine Endometrium during Maternal Recognition of Pregnancy
| Autor: | Ted Kalbfleisch, Ann M. Hess, Gerrit J. Bouma, S.J. Coleman, Milton G Thomas, Juan F. Medrano, Alma Islas-Trejo, Jason E. Bruemmer, Kristin M. Klohonatz |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Small RNA lcsh:QH426-470 1.1 Normal biological development and functioning maternal recognition of pregnancy Reproductive health and childbirth Biology Luteal phase Endometrium Article Andrology Transcriptome 03 medical and health sciences 0302 clinical medicine stomatognathic system Clinical Research Pregnancy Genetics medicine Animals Horses Small nucleolar RNA Genetics (clinical) reproductive and urinary physiology equine Animal Sequence Analysis RNA urogenital system Contraception/Reproduction RNA Non-coding RNA lcsh:Genetics 030104 developmental biology medicine.anatomical_structure Pregnancy Animal Female pregnancy Sequence Analysis transcriptome 030217 neurology & neurosurgery Small nuclear RNA |
| Zdroj: | Genes Volume 10 Issue 10 Genes, vol 10, iss 10 Genes, Vol 10, Iss 10, p 749 (2019) |
| ISSN: | 2073-4425 |
| DOI: | 10.3390/genes10100749 |
| Popis: | Equine maternal recognition of pregnancy (MRP) is a process whose signal remains unknown. During MRP the conceptus and endometrium communicate to attenuate prostaglandin F2&alpha (PGF) secretion, sparing the corpus luteum and maintaining progesterone production. Recognition of a mobile conceptus by the endometrium is critical by days 14&ndash 16 post-ovulation (PO), when endometrium produces PGF, initiating luteolysis. The objective of this study was to evaluate endometrial gene expression changes based upon pregnancy status via RNA sequencing. This experiment utilized a cross-over design with each mare serving as both a pregnant and non-mated control on days nine, 11, and 13 PO (n = 3/status/day). Mares were randomly assigned to collection day and pregnancy confirmed by terminal uterine lavage at the time of endometrial biopsy. Total RNA was isolated and libraries prepared using Illumina TruSeq RNA sample preparation kit. Reads were mapped and annotated using HISAT2 and Stringtie. Expression values were evaluated with DESEQ2 (P &le 0.05 indicated significance). On day nine, 11, and 13 there were 1435, 1435 and 916 significant transcripts, respectively. Multiple genes with splice variants had different expression patterns within the same day. These are the first data to evaluate the endometrial transcriptome during MRP on days nine, 11, and 13. |
| Databáze: | OpenAIRE |
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