Effect of desiccating stress on mouse meibomian gland function
| Autor: | Jeffrey L, Suhalim, Geraint J, Parfitt, Yilu, Xie, Cintia S, De Paiva, Cintia S, De Pavia, Stephen C, Pflugfelder, Tejas N, Shah, Eric O, Potma, Donald J, Brown, James V, Jester |
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| Rok vydání: | 2014 |
| Předmět: |
Pathology
Dry Eye Syndromes Meibomian gland Spectrum Analysis Raman Inbred C57BL Ophthalmology & Optometry Cholinergic Antagonists Basal (phylogenetics) Mice evaporative dry eye Raman Acinar cell proliferation biology meibomian gland Meibomian Glands nonlinear optical microscopy medicine.anatomical_structure Female Antibody medicine.medical_specialty Physiological 1.1 Normal biological development and functioning Scopolamine Stress Article Andrology Immune system Stress Physiological Underpinning research Opthalmology and Optometry medicine Animals Desiccation Eye Proteins Eye Disease and Disorders of Vision Cell Proliferation Cell growth Animal Spectrum Analysis Lipid metabolism Humidity Lipid Metabolism Mice Inbred C57BL Ophthalmology Disease Models Animal Disease Models biology.protein stimulated Raman scattering sense organs |
| Zdroj: | The ocular surface, vol 12, iss 1 |
| Popis: | Purpose Mice exposed to standardized desiccating environmental stress to induce dry eye-like symptoms have been used as a model to study the underlying mechanisms of evaporative dry eye. While studies have shown marked inflammatory and immune changes, the effect of such stress on meibomian gland function remains largely unknown. We sought to evaluate the effects of desiccating stress on meibocyte proliferation and meibum quality. Methods Ten mice were treated with scopolamine and subjected to a drafty low humidity environment (30-35%). Five and ten days after treatment, eyelids were harvested and cryosections stained with Ki67 antibody to identify cycling cells. Sections were also imaged using stimulated Raman scattering (SRS) microscopy to characterize the gland compositional changes by detecting the vibrational signatures of methylene (lipid) and amide-I (protein). Results Desiccating stress caused a 3-fold increase in basal acinar cell proliferation from 18.3 ± 11.1% in untreated mice to 64.4 ± 19.9% and 66.6 ± 13.4% after 5 and 10 days exposure, respectively (P < .001). In addition, SRS analysis showed a wider variation in the protein-to-lipid ratio throughout the gland, suggesting alterations in meibocyte differentiation and lipid synthesis. Conclusions These data are consistent with a model that a desiccating environment may have a direct effect on meibomian gland function, leading to a significant increase in basal acinar cell proliferation, abnormal meibocyte differentiation, and altered lipid production. |
| Databáze: | OpenAIRE |
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