A functional tricarboxylic acid cycle operates during growth of Bordetella pertussis on amino acid mixtures as sole carbon substrates
| Autor: | Dominique Garnier, Denis Speck, Nic D. Lindley, Marie Izac |
|---|---|
| Přispěvatelé: | Izac, Marie, Lindley, Nicholas, Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Sanofi Pasteur SA, Sanofi-Pasteur, Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Bordetella pertussis
[SDV]Life Sciences [q-bio] Citric Acid Cycle acide tricarboxylique lcsh:Medicine Citrate (si)-Synthase Aconitase 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Citrate synthase substrat carbone Amino Acids lcsh:Science 030304 developmental biology chemistry.chemical_classification 0303 health sciences Multidisciplinary biology 030306 microbiology lcsh:R bordetella pertussis biology.organism_classification Isocitrate Dehydrogenase Amino acid Culture Media Citric acid cycle Kinetics acide aminé Isocitrate dehydrogenase Biochemistry chemistry biology.protein Carbohydrate Metabolism lcsh:Q Citric acid Transcriptome amino acid Genome Bacterial tricarboxylic acid Research Article |
| Zdroj: | Plos One 12 (10), . (2015) PLoS ONE PLoS ONE, 2015, 10 (12), ⟨10.1371/journal.pone.0145251⟩ PLoS ONE, Public Library of Science, 2015, 10 (12), ⟨10.1371/journal.pone.0145251⟩ PLoS ONE, Vol 10, Iss 12, p e0145251 (2015) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0145251⟩ |
| Popis: | It has been claimed that citrate synthase, aconitase and isocitrate dehydrogenase activities are non-functional in Bordetella pertussis and that this might explain why this bacterium's growth is sometimes associated with accumulation of polyhydroxybutyrate (PHB) and/or free fatty acids. However, the sequenced genome includes the entire citric acid pathway genes. Furthermore, these genes were expressed and the corresponding enzyme activities detected at high levels for the pathway when grown on a defined medium imitating the amino acid content of complex media often used for growth of this pathogenic micro-organism. In addition, no significant PHB or fatty acids could be detected. Analysis of the carbon balance and stoichiometric flux analysis based on specific rates of amino acid consumption, and estimated biomass requirements coherent with the observed growth rate, clearly indicate that a fully functional tricarboxylic acid cycle operates in contrast to previous reports. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|