Functional interactions between alternatively spliced forms of Pax6 in crystallin gene regulation and in haploinsufficiency
| Autor: | Bharesh K. Chauhan, Ales Cvekl, K. Cveklova, Ying Yang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Transcriptional Activation
endocrine system Heterozygote PAX6 Transcription Factor PAX5 Transcription Factor Biology DNA-binding protein Cell Line Mice Genetics Animals Humans Paired Box Transcription Factors gamma-Crystallins Eye Proteins Transcription factor Regulation of gene expression Homeodomain Proteins Alternative splicing alpha-Crystallin B Chain Promoter Articles Crystallins eye diseases DNA-Binding Proteins Repressor Proteins Alternative Splicing Mutation PAX6 sense organs Haploinsufficiency Transcription Factors |
| Popis: | Pax6 is essential for development of the eye, olfactory system, brain and pancreas. Haploinsufficiency of Pax6 causes abnormal eye development. Two forms of Pax6 protein, PAX6 and PAX6(5a), differ in a 14 amino acid insertion encoded by an alternatively spliced exon 5a in the N-terminal DNA-binding paired domain (PD), and they are simultaneously expressed. Here, we show that PAX6 and PAX6(5a) together synergistically activate transcription from promoters recognized by Pax6 PD and PD5a, but not by their homeodomain. This synergism promotes activation of transcription by c-Maf and MafA on the alphaB-crystallin promoter, and is required for transcriptional co-activation by RARbeta/RXRbeta and PAX6/PAX6(5a) on the gammaF-crystallin promoter. To determine the role of this synergism in haploinsufficiency, we tested four human missense (G18W, R26G, G64V and R128C) and one nonsense (R317X) mutants, with reporters driven by Pax6 PD consensus binding sites and the alphaB-crystallin promoter. The simultaneous activity of Pax6 proteins [PAX6, mutated PAX6, PAX6(5a) and mutated PAX6(5a)] modeling haploinsufficiency yielded results not predicted by properties of individual PAX6 or PAX6(5a). Taken together, these results indicate that complex ocular phenotypes due to Pax6 haploinsufficiency originate, at least partially, from functional interactions between alternatively spliced PAX6 and PAX6(5a) variants and other factors, e.g. MafA/c-Maf. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|