A Panel of Serum MiRNA Biomarkers for the Diagnosis of Severe to Mild Traumatic Brain Injury in Humans
| Autor: | Kevin K.W. Wang, Radha K. Maheshwari, Manish Bhomia, Linda Papa, Nagaraja S. Balakathiresan |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Male 0301 basic medicine Traumatic brain injury Bioinformatics Severity of Illness Index Article 03 medical and health sciences 0302 clinical medicine Cerebrospinal fluid Brain Injuries Traumatic Severity of illness microRNA TaqMan Humans Medicine Multidisciplinary business.industry Gene Expression Profiling Case-control study medicine.disease 3. Good health Gene expression profiling MicroRNAs 030104 developmental biology Real-time polymerase chain reaction ROC Curve Case-Control Studies Female business Biomarkers 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | MicroRNAs (MiRNAs) are small endogenous RNA molecules and have emerged as novel serum diagnostic biomarkers for several diseases due to their stability and detection at minute quantities. In this study, we have identified a serum miRNA signature in human serum samples of mild to severe TBI, which can be used for diagnosis of mild and moderate TBI (MMTBI). Human serum samples of MMTBI, severe TBI (STBI), orthopedic injury and healthy controls were used and miRNA profiling was done using taqman real time PCR. The real time PCR data for the MMTBI, STBI and orthopedic injury was normalized to the control samples which showed upregulation of 39, 37 and 33 miRNAs in MMTBI, STBI and orthopedic injury groups respectively. TBI groups were compared to orthopedic injury group and an up-regulation of 18 and 20 miRNAs in MMTBI and STBI groups was observed. Among these, a signature of 10 miRNAs was found to be present in both MMTBI and STBI groups. These 10 miRNAs were validated in cerebrospinal fluid (CSF) from STBI and four miRNAs were found to be upregulated in CSF. In conclusion, we identified a subset of 10 unique miRNAs which can be used for diagnosis of MMTBI and STBI. |
| Databáze: | OpenAIRE |
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