Widespread Differential Expression of Coding Region and 3′ UTR Sequences in Neurons and Other Tissues
| Autor: | Terence Duarte, Mary Hynes, Saranya Santhosh Kumar, Arif Kocabas |
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| Jazyk: | angličtina |
| Předmět: |
Male
Neurons Genetics Untranslated region Regulation of gene expression Messenger RNA Base Sequence Three prime untranslated region Neuroscience(all) General Neuroscience RNA Mice Transgenic Biology Mice Inbred C57BL Mice Open Reading Frames Gene Expression Regulation Gene expression NIH 3T3 Cells Animals Coding region RNA Messenger 3' Untranslated Regions Gene |
| Zdroj: | Neuron. (6):1149-1156 |
| ISSN: | 0896-6273 |
| DOI: | 10.1016/j.neuron.2015.10.048 |
| Popis: | SummaryMature messenger RNAs (mRNAs) consist of coding sequence (CDS) and 5′ and 3′ UTRs, typically expected to show similar abundance within a given neuron. Examining mRNA from defined neurons, we unexpectedly show extremely common unbalanced expression of cognate 3′ UTR and CDS sequences; many genes show high 3′ UTR relative to CDS, others show high CDS to 3′ UTR. In situ hybridization (19 of 19 genes) shows a broad range of 3′ UTR-to-CDS expression ratios across neurons and tissues. Ratios may be spatially graded or change with developmental age but are consistent across animals. Further, for two genes examined, a 3′ UTR-to-CDS ratio above a particular threshold in any given neuron correlated with reduced or undetectable protein expression. Our findings raise questions about the role of isolated 3′ UTR sequences in regulation of protein expression and highlight the importance of separately examining 3′ UTR and CDS sequences in gene expression analyses. |
| Databáze: | OpenAIRE |
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