Cyclic nucleotide-specific phosphodiesterases of Plasmodium falciparum: PfPDEα, a non-essential cGMP-specific PDE that is an integral membrane protein
| Autor: | Juergen Klar, Hermann Tenor, Thomas Seebeck, Reto Brun, Selina Bopp, Hans-Peter Beck, Laurent Wentzinger |
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| Rok vydání: | 2008 |
| Předmět: |
biology
Phosphoric Diester Hydrolases Molecular Sequence Data Plasmodium falciparum Alternative splicing Protozoan Proteins Membrane Proteins Phosphodiesterase Primary transcript biology.organism_classification Polymerase Chain Reaction Gene Expression Regulation Enzymologic Malaria Blotting Southern Transmembrane domain Infectious Diseases Biochemistry Membrane protein Animals Parasitology Enzyme Inhibitors Cyclic GMP Gene Integral membrane protein |
| Zdroj: | International Journal for Parasitology. 38:1625-1637 |
| ISSN: | 0020-7519 |
| Popis: | Cyclic nucleotide-specific phosphodiesterases (PDEs) have come into focus as interesting potential targets for PDE inhibitor-based anti-parasitic drugs. Genomes of the various agents of human malaria, most notably Plasmodium falciparum, all contain four genes for class 1 PDEs. The catalytic domains of these enzymes are closely related to those of the 11 human PDE families. This presents the possibility that the available vast expertise in developing drugs against human PDEs might now also be applied to developing compounds that are active against malarial PDEs. The current study identifies four Plasmodium genes that code for PfPDEalpha, PfPDEbeta, PfPDEgamma and PfPDEdelta, respectively. It further demonstrates that the PfPDEalpha polypeptide exists in two versions (PfPDEalphaA and PfPDEalphaB) that are generated by alternative splicing of the primary transcript. All malarial PDEs contain several transmembrane helices in their N-terminal regions, indicating that they are integral membrane proteins. In agreement with this prediction, essentially all PDE activity is associated with the cell membranes. PfPDEalpha was characterized as a cGMP-specific PDE that is not sensitive to a number of standard PDE inhibitors. Genetic ablation of the PfPDE1 gene produced no major phenotype in erythrocyte cultures. |
| Databáze: | OpenAIRE |
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