Molecular and Biochemical Characterization of the Natural Chromosome-Encoded Class A -Lactamase from Pseudomonas luteola
| Autor: | I. Casin, François-Xavier Weill, Benoît Doublet, Frédéric Robin, R. Bonnet, Laetitia Fabre, Anne Le Fleche |
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| Přispěvatelé: | Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Centre National de Référence des Salmonella - Bactéries pathogènes entériques (CNR), Institut Pasteur [Paris] (IP), CHU Clermont-Ferrand, Université de Clermont-Ferrand, Hopital Saint-Louis [AP-HP] (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Biodiversité des Bactéries Pathogènes Émergentes, B.D. was supported by an INRA postdoctoral fellowship., Infectiologie Animale et Santé Publique - IASP (Nouzilly, France), Centre National de Référence des Salmonella-Bactéries pathogènes entériques (CNR), Institut Pasteur [Paris], Hopital Saint-Louis, Assistance Publique – Hôpitaux de Paris (AP-HP) |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
bet-lactamase
MESH: beta-Lactamases MESH: Amino Acid Sequence medicine.disease_cause Genetic analysis Substrate Specificity MESH: Recombinant Proteins Catheters Indwelling Ralstonia MESH: Reverse Transcriptase Polymerase Chain Reaction Pharmacology (medical) MESH: Genetic Variation Cloning Molecular MESH: Phylogeny Phylogeny Genetics 0303 health sciences MESH: Microbial Sensitivity Tests biology MESH: Kinetics Reverse Transcriptase Polymerase Chain Reaction Nucleic acid sequence MESH: Pseudomonas Infections Chromosomes Bacterial Recombinant Proteins 3. Good health Anti-Bacterial Agents Electrophoresis Gel Pulsed-Field Infectious Diseases MESH: Electrophoresis Gel Pulsed-Field MESH: Isoelectric Focusing MESH: Genes Bacterial Plasmids MESH: Chromosomes Bacterial Molecular Sequence Data MESH: Catheters Indwelling Microbial Sensitivity Tests Molecular cloning beta-Lactamases Microbiology 03 medical and health sciences Mechanisms of Resistance MESH: Plasmids Pseudomonas MESH: Anti-Bacterial Agents medicine Citrobacter sedlakii Pseudomonas Infections MESH: Cloning Molecular Amino Acid Sequence Gene Escherichia coli Pseudomonas luteola 030304 developmental biology Pharmacology [SDV.GEN]Life Sciences [q-bio]/Genetics MESH: Molecular Sequence Data 030306 microbiology Genetic Variation MESH: Pseudomonas biochemical phenomena metabolism and nutrition biology.organism_classification bacterial infections and mycoses [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Kinetics Genes Bacterial MESH: Substrate Specificity Isoelectric Focusing |
| Zdroj: | Antimicrobial Agents and Chemotherapy Antimicrobial Agents and Chemotherapy, 2010, 54 (1), pp.45-51. ⟨10.1128/AAC.00427-09⟩ Antimicrobial Agents and Chemotherapy, American Society for Microbiology, 2010, 54 (1), pp.45-51. ⟨10.1128/AAC.00427-09⟩ |
| ISSN: | 0066-4804 1098-6596 |
| Popis: | Pseudomonas luteola (formerly classified as CDC group Ve-1 and named Chryseomonas luteola ) is an unusual pathogen implicated in rare but serious infections in humans. A novel β-lactamase gene, bla LUT-1 , was cloned from the whole-cell DNA of the P. luteola clinical isolate LAM, which had a weak narrow-spectrum β-lactam-resistant phenotype, and expressed in Escherichia coli . This gene encoded LUT-1, a 296-amino-acid Ambler class A β-lactamase with a pI of 6 and a theoretical molecular mass of 28.9 kDa. The catalytic efficiency of this enzyme was higher for cephalothin, cefuroxime, and cefotaxime than for penicillins. It was found to be 49% to 59% identical to other Ambler class A β-lactamases from Burkholderia sp. (PenA to PenL), Ralstonia eutropha (REUT), Citrobacter sedlakii (SED-1), Serratia fonticola (FONA and SFC-1), Klebsiella sp. (KPC and OXY), and CTX-M extended-spectrum β-lactamases. No gene homologous to the regulatory ampR genes of class A β-lactamases was found in the vicinity of the bla LUT-1 gene. The entire bla LUT-1 coding region was amplified by PCR and sequenced in five other genetically unrelated P. luteola strains (including the P. luteola type strain). A new variant of bla LUT-1 was found for each strain. These genes (named bla LUT-2 to bla LUT-6 ) had nucleotide sequences 98.1 to 99.5% identical to that of bla LUT-1 and differing from this gene by two to four nonsynonymous single nucleotide polymorphisms. The bla LUT gene was located on a 700- to 800-kb chromosomal I-CeuI fragment, the precise size of this fragment depending on the P. luteola strain. |
| Databáze: | OpenAIRE |
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