Identification of tissue inhibitor of metalloproteinase-2 (TIMP-2)-progelatinase complex as the third metalloproteinase inhibitor peak in rheumatoid synovial fluid
Autor: | Tim E. Cawston, Brian L. Hazleman, H F Bigg, Ian M. Clark |
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Rok vydání: | 1993 |
Předmět: |
Knee Joint
Immunoblotting Immunology Connective tissue Matrix metalloproteinase General Biochemistry Genetics and Molecular Biology Arthritis Rheumatoid Extracellular matrix alpha-2-Macroglobulin Rheumatology Multienzyme Complexes Synovial Fluid medicine Humans Immunology and Allergy Synovial fluid Enzyme Precursors Tissue Inhibitor of Metalloproteinase-2 Metalloproteinase biology business.industry Metalloendopeptidases Tissue inhibitor of metalloproteinase Neoplasm Proteins medicine.anatomical_structure Biochemistry Gelatinases Enzyme inhibitor Chromatography Gel biology.protein Electrophoresis Polyacrylamide Gel business Research Article |
Zdroj: | Annals of the Rheumatic Diseases. 52:177-181 |
ISSN: | 0003-4967 |
Popis: | The metalloproteinases are a family of enzymes that can degrade all the components of the extracellular matrix. These potent enzymes are often found in proenzyme forms and require activation before the substrate can be digested. To prevent unlimited connective tissue destruction a number of inhibitors exist to limit their activity. In a previous study it was found that metalloproteinases in proenzyme form and metalloproteinase inhibitors were often present in rheumatoid synovial fluids. Two of these inhibitors were identified in rheumatoid synovial fluid as alpha 2 macroglobulin and tissue inhibitor of metalloproteinase (TIMP), the specific metalloproteinase inhibitor. A third inhibitory peak was unidentified. In the study reported here it was shown that this third inhibitor can be purified using gelatin-Sepharose chromatography and consists of TIMP-2 bound to progelatinase (relative molecular weight 72,000) in a similar way to that found in concentrated connective tissue culture medium. The importance of these proteinase inhibitors in synovial fluid is discussed. |
Databáze: | OpenAIRE |
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