Micropillar array chip for integrated white blood cell isolation and PCR
| Autor: | Xing Jian Lou, Paolo Fortina, Nicholas J. Panaro, Larry J. Kricka, Peter Wilding |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Materials science
microfluidic modeling polymerase chain reaction Microfluidics blood cell isolation Bioengineering Cell Separation Sensitivity and Specificity Capillary electrophoresis White blood cell Leukocytes medicine Humans Molecular Biology micropillars Whole blood Chromatography Human blood Extramural Reproducibility of Results Equipment Design Microfluidic Analytical Techniques Amplicon Flow Cytometry Chip Molecular biology Equipment Failure Analysis medicine.anatomical_structure Biotechnology |
| Zdroj: | Biomolecular Engineering. 21:157-162 |
| ISSN: | 1389-0344 |
| DOI: | 10.1016/j.bioeng.2004.11.001 |
| Popis: | We report the fabrication of silicon chips containing a row of 667 pillars, 10 by 20 microm in cross-section, etched to a depth of 80 microm with adjacent pillars being separated by 3.5 microm. The chips were used to separate white blood cells from whole blood in less than 2 min and for subsequent PCR of a genomic target (eNOS). Chip fluid dynamics were validated experimentally using CoventorWare microfluidic simulation software. The amplicon concentrations were determined using microchip capillary electrophoresis and were >40% of that observed in conventional PCR tubes for chips with and without pillars. Reproducible on-chip PCR was achieved using white blood cell preparations isolated from whole human blood pumped through the chip. |
| Databáze: | OpenAIRE |
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